Periodic concentration-polarization based formation of biomolecule preconcentration

Sinwook Park, Ramadan Abu-Rjal, Keren Buchnik, Yechezkel Kashi, Gilad Yossifon

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Ionic concentration-polarization (CP)-based biomolecule preconcentration is an established method for enhancing the detection sensitivity of target biomolecules immunoassay. However, the formed preconcentrated plug of biomolecules sweeps over the surface immobilized antibodies and results in a short duration of overlap and with this also a smaller binding between target analyte and immobilized molecular probes. To overcome this, we have periodically formed a preconcentrated plug of biomolecules sweeping over the desired region of binding. We found that there is an optimal sweeping condition which results in maximum retention time of the preconcentrated plug over a desired sensing region and enhanced detection sensitivity.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages66-67
Number of pages2
ISBN (Electronic)9781733419017
StatePublished - 1 Jan 2020
Externally publishedYes
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: 4 Oct 20209 Oct 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period4/10/209/10/20

Keywords

  • Human IgG
  • Ion concentration polarization (CP)
  • Preconcentration
  • Sandwich immunoassay

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