Permanent genome modifications in plant cells by transient viral vectors

Alexander Vainstein, Ira Marton, Amir Zuker, Micha Danziger, Tzvi Tzfira

Research output: Contribution to journalReview articlepeer-review

20 Scopus citations

Abstract

Endonuclease-mediated induction of genomic double-strand breaks has enabled genome editing in living cells. However, deploying this technology for the induction of gene disruption in plant cells often relies on direct gene transfer of endonuclease (i.e. zinc finger nuclease or homing endonuclease) expression constructs into the targeted cell, followed by regeneration of a mutated plant. Such mutants, even when they have no detectable traces of foreign DNA, might still be classified as transgenic because of the transgenic nature of the endonuclease delivery method. Indirect delivery of endonucleases into target cells by viral vectors provides a unique non-transgenic approach to the production of mutated plants. Furthermore, viral vectors can spread into the growing and developing tissues of infected plants, which could provide a unique opportunity to bypass the regeneration step that is often required in direct gene-transfer methods.

Original languageEnglish
Pages (from-to)363-369
Number of pages7
JournalTrends in Biotechnology
Volume29
Issue number8
DOIs
StatePublished - 1 Aug 2011

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

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