Peroxidase activity as a marker for estrogenicity: Studies in uterus and mammary tumors

J. Levy, Y. Liel, S. M. Glick

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We examined the possibility that peroxidase activity might be a marker for estrogen activity in established estrogen-dependent tissues: dimethylbenz[a]anthracene (DMBA)-induced rat mammary tumors and human breast cancer. In DMBA-induced tumors undergoing regression after ovariectomy or tamoxifen treatment, tumor size decreased by 50%, estradiol receptors (ER) and progesterone receptors (PgR) decreased by 25 and 20%, respectively, but peroxidase activity paradoxically increased six- to sevenfold. In DMBA tumors stimulated by estradiol treatment or by the cessation of tamoxifen administration in intact rats, tumor size increased threefold. ER and PgR increased two- and threefold, respectively, while peroxidase activity decreased 50%. These data indicate an inverse relation between tumor growth, ER and PgR on the one hand, and peroxidase activity on the other. In the human breast cancers there was a significant negative activity on the other. In the human breast cancers there was a significant negative relation between the presence of ER and peroxidase activity. By using a calibrated Sephadex G-100 column it was shown that uterine peroxidase differs in molecular weight from the peroxidase of rat mammary tumors and that of human breast cancer.

Original languageEnglish
Pages (from-to)970-975
Number of pages6
JournalIsrael Journal of Medical Sciences
Volume17
Issue number9-10
StatePublished - 1 Dec 1981
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering

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