Perpendicular planes of FtsZ arcs in spheroidal Escherichia coli cells

Evelien Pas; Monica Einav; Conrad L. Woldringh; Arieh Zaritsky

doi:10.1016/S0300-9084(00)01219-0

Perpendicular planes of FtsZ arcs in spheroidal Escherichia coli cells

Evelien Pas, Monica Einav, Conrad L. Woldringh, Arieh Zaritsky

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Division planes in Escherichia coli, usually restricted to one dimension of the rod-shaped cell, were induced at all possible planes by transforming the cells to spheroids with mecillinam (inactivating PbpA). Such cells displayed many nucleoids and arcs of FtsZ, genetically tagged to green fluorescent protein, that developed to rings at constriction sites all around their surface. These observations are consistent with the view (Woldringh et al., J. Bacteriol. 176 (1994) 6030-6038) that nucleoids, forced during replication to segregate in the length axis of the cell by the rigid bacillary envelope, induce assembly of FtsZ to division rings in between them.

Original language	English
Pages (from-to)	121-124
Number of pages	4
Journal	Biochimie
Volume	83
Issue number	1
DOIs	https://doi.org/10.1016/S0300-9084(00)01219-0
State	Published - 1 Jan 2001

Keywords

Division planes
Fluorescent microscopy
FtsZ-GFP rings and arcs
Mecillinam-induced spheroidal cells
Nucleoid segregation
Thymine limitation

ASJC Scopus subject areas

Biochemistry

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10.1016/S0300-9084(00)01219-0

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title = "Perpendicular planes of FtsZ arcs in spheroidal Escherichia coli cells",

abstract = "Division planes in Escherichia coli, usually restricted to one dimension of the rod-shaped cell, were induced at all possible planes by transforming the cells to spheroids with mecillinam (inactivating PbpA). Such cells displayed many nucleoids and arcs of FtsZ, genetically tagged to green fluorescent protein, that developed to rings at constriction sites all around their surface. These observations are consistent with the view (Woldringh et al., J. Bacteriol. 176 (1994) 6030-6038) that nucleoids, forced during replication to segregate in the length axis of the cell by the rigid bacillary envelope, induce assembly of FtsZ to division rings in between them.",

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T1 - Perpendicular planes of FtsZ arcs in spheroidal Escherichia coli cells

AU - Pas, Evelien

AU - Einav, Monica

AU - Woldringh, Conrad L.

AU - Zaritsky, Arieh

PY - 2001/1/1

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N2 - Division planes in Escherichia coli, usually restricted to one dimension of the rod-shaped cell, were induced at all possible planes by transforming the cells to spheroids with mecillinam (inactivating PbpA). Such cells displayed many nucleoids and arcs of FtsZ, genetically tagged to green fluorescent protein, that developed to rings at constriction sites all around their surface. These observations are consistent with the view (Woldringh et al., J. Bacteriol. 176 (1994) 6030-6038) that nucleoids, forced during replication to segregate in the length axis of the cell by the rigid bacillary envelope, induce assembly of FtsZ to division rings in between them.

AB - Division planes in Escherichia coli, usually restricted to one dimension of the rod-shaped cell, were induced at all possible planes by transforming the cells to spheroids with mecillinam (inactivating PbpA). Such cells displayed many nucleoids and arcs of FtsZ, genetically tagged to green fluorescent protein, that developed to rings at constriction sites all around their surface. These observations are consistent with the view (Woldringh et al., J. Bacteriol. 176 (1994) 6030-6038) that nucleoids, forced during replication to segregate in the length axis of the cell by the rigid bacillary envelope, induce assembly of FtsZ to division rings in between them.

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KW - Fluorescent microscopy

KW - FtsZ-GFP rings and arcs

KW - Mecillinam-induced spheroidal cells

KW - Nucleoid segregation

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Perpendicular planes of FtsZ arcs in spheroidal Escherichia coli cells

Abstract

Keywords

ASJC Scopus subject areas

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