Perpendicular planes of FtsZ arcs in spheroidal Escherichia coli cells

Evelien Pas, Monica Einav, Conrad L. Woldringh, Arieh Zaritsky

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Division planes in Escherichia coli, usually restricted to one dimension of the rod-shaped cell, were induced at all possible planes by transforming the cells to spheroids with mecillinam (inactivating PbpA). Such cells displayed many nucleoids and arcs of FtsZ, genetically tagged to green fluorescent protein, that developed to rings at constriction sites all around their surface. These observations are consistent with the view (Woldringh et al., J. Bacteriol. 176 (1994) 6030-6038) that nucleoids, forced during replication to segregate in the length axis of the cell by the rigid bacillary envelope, induce assembly of FtsZ to division rings in between them.

Original languageEnglish
Pages (from-to)121-124
Number of pages4
JournalBiochimie
Volume83
Issue number1
DOIs
StatePublished - 1 Jan 2001

Keywords

  • Division planes
  • Fluorescent microscopy
  • FtsZ-GFP rings and arcs
  • Mecillinam-induced spheroidal cells
  • Nucleoid segregation
  • Thymine limitation

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