TY - JOUR
T1 - Persistent viral infection affects tumorigenicity of a neuroblastoma cell line
AU - Schattner, A.
AU - Rager-Zisman, B.
AU - Bloom, B. R.
N1 - Funding Information:
’ Supported by U.S. Public Health Service Grant A109807, Grant 1006 from the National Multiple Sclerosis Society, and Grant ACBC4390 from the American Cancer Society. 2 Fellow of the Camp David Institute for International Health. 3 Present Address: Department of Microbiology and Immunology, Faculty of Health Sciences, Ben Gurion University, EteerS heva, Israel 4 To whom correspondence should be sent. 5 Abbreviations used: NSZOY, mouse neuroblastoma cell line; NSZOY/MS, persistently infected with measlesv irus; PI, persistently infected, NK, natural killer cells; IFN, interferon; EOP, efficiency of plating; FCS, fetal calf serum; sc, subcutaneously; iv, intravenously.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - A mouse neuroblastoma cell line (clone NS20Y) is highly tumorigenic in syngeneic A/J mice. When this clone was persistently infected with measles virus (NS20Y/MS) it failed to grow or form tumors in conventional A/J or nude mice, even when large numbers of cells were inoculated. As doubling time, serum dependence, and anchorage-independent growth on agar did not differ significantly between NS20Y and NS20Y/MS, lack of tumorigenicity of the persistently infected cells is unlikely to be due to an intrinsic property of the cells. NS20Y/MS cells were found to be effectively rejected in athymic nude as well as conventional syngeneic mice. However, injection of mice with either anti-interferon or anti-asialo GM1 serum, both of which have been shown to deplete natural killer (NK) cells in vivo, enabled NS20Y/MS cells to form large tumors. Unexpectedly, treatment of mice with silica also allowed the NS20Y/MS cells to form tumors. Under these conditions, it was shown that silica caused a significant decrease in NK activity as late as 7 days after a single injection. Although NS20Y/MS were not susceptible to NK cell lysis in vitro, the in vivo data suggest that NK cells are in fact the prime mechanism in the rejection of this persistently virus-infected neuroblastoma cell line by athymic and conventional syngeneic mice. The results indicate that NK activity may be greater or more sensitively detected in vivo than in vitro.
AB - A mouse neuroblastoma cell line (clone NS20Y) is highly tumorigenic in syngeneic A/J mice. When this clone was persistently infected with measles virus (NS20Y/MS) it failed to grow or form tumors in conventional A/J or nude mice, even when large numbers of cells were inoculated. As doubling time, serum dependence, and anchorage-independent growth on agar did not differ significantly between NS20Y and NS20Y/MS, lack of tumorigenicity of the persistently infected cells is unlikely to be due to an intrinsic property of the cells. NS20Y/MS cells were found to be effectively rejected in athymic nude as well as conventional syngeneic mice. However, injection of mice with either anti-interferon or anti-asialo GM1 serum, both of which have been shown to deplete natural killer (NK) cells in vivo, enabled NS20Y/MS cells to form large tumors. Unexpectedly, treatment of mice with silica also allowed the NS20Y/MS cells to form tumors. Under these conditions, it was shown that silica caused a significant decrease in NK activity as late as 7 days after a single injection. Although NS20Y/MS were not susceptible to NK cell lysis in vitro, the in vivo data suggest that NK cells are in fact the prime mechanism in the rejection of this persistently virus-infected neuroblastoma cell line by athymic and conventional syngeneic mice. The results indicate that NK activity may be greater or more sensitively detected in vivo than in vitro.
UR - http://www.scopus.com/inward/record.url?scp=0021955596&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(85)90173-X
DO - 10.1016/0008-8749(85)90173-X
M3 - Article
AN - SCOPUS:0021955596
SN - 0008-8749
VL - 90
SP - 103
EP - 114
JO - Cellular Immunology
JF - Cellular Immunology
IS - 1
ER -
