Abstract
The article describes basics of approach to build a device dedicated to sensitive assessment of phagocyte activity in biological fluids. The unique place of phagocytosis in the innate immune system, the mechanisms, localization and factors of oxidative phagocyte activity as well as the techniques used to detect respiratory burst and localization are briefly reviewed. Sensor design combines simplicity and artifact -absence in whole blood system, simultaneous record and analysis of extra-cellular and intra-cellular luminescence, sensitivity and efficiency of signal capturing of optical fiber–photomultiplier detection system and the convenience of disposable sample holder.
| Original language | English |
|---|---|
| Title of host publication | Handbook of Biosensors and Biochips |
| Subtitle of host publication | Cullen/Biosensors |
| Publisher | wiley |
| Pages | 1-19 |
| Number of pages | 19 |
| ISBN (Electronic) | 9780470061565 |
| ISBN (Print) | 9780470019054 |
| DOIs | |
| State | Published - 1 Jan 2008 |
Keywords
- blood
- chemiluminescence
- disposable
- extracellular
- fiber
- intracellular
- luminometer
- phagocyte
- sensor
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- General Chemistry