Phenotypic heterogeneity in 3-methylcholanthrene - Induced transformation of normal rat kidney cells infected with Moloney murine leukemia virus

Yehudith Hassan; Marina Wolfson; Mordechai Aboud

doi:10.1016/0309-1651(86)90015-9

Phenotypic heterogeneity in 3-methylcholanthrene - Induced transformation of normal rat kidney cells infected with Moloney murine leukemia virus

Yehudith Hassan, Marina Wolfson, Mordechai Aboud

The Shraga Segal Department of Microbiology, Immunology and Genetics

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Normal rat kidney cells, infected with Moloney murine leukemia virus, were treated with 3-methylcholanthrene at passage 5 postinfection. Foci of transformed cells appeared after 9-11 passages following this treatment. Characterization of four different randomly isolated foci revealed a striking diversity with respect to various tested phenotypic parameters. Remarkable differences were observed among these transformed clones regarding their cell morphology, growth rate, saturation density, serum requirements, virus release and its response to rat and mouse fibroblast interferons. This study demonstrates that cell transformation by chemical-retroviral co-carcinogenesis may lead to the formation of phenotypically heterogenous tumor cells.

Original language	English
Pages (from-to)	19-26
Number of pages	8
Journal	Cell Biology International Reports
Volume	10
Issue number	1
DOIs	https://doi.org/10.1016/0309-1651(86)90015-9
State	Published - 1 Jan 1986

ASJC Scopus subject areas

Cell Biology

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10.1016/0309-1651(86)90015-9

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abstract = "Normal rat kidney cells, infected with Moloney murine leukemia virus, were treated with 3-methylcholanthrene at passage 5 postinfection. Foci of transformed cells appeared after 9-11 passages following this treatment. Characterization of four different randomly isolated foci revealed a striking diversity with respect to various tested phenotypic parameters. Remarkable differences were observed among these transformed clones regarding their cell morphology, growth rate, saturation density, serum requirements, virus release and its response to rat and mouse fibroblast interferons. This study demonstrates that cell transformation by chemical-retroviral co-carcinogenesis may lead to the formation of phenotypically heterogenous tumor cells.",

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AU - Aboud, Mordechai

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AB - Normal rat kidney cells, infected with Moloney murine leukemia virus, were treated with 3-methylcholanthrene at passage 5 postinfection. Foci of transformed cells appeared after 9-11 passages following this treatment. Characterization of four different randomly isolated foci revealed a striking diversity with respect to various tested phenotypic parameters. Remarkable differences were observed among these transformed clones regarding their cell morphology, growth rate, saturation density, serum requirements, virus release and its response to rat and mouse fibroblast interferons. This study demonstrates that cell transformation by chemical-retroviral co-carcinogenesis may lead to the formation of phenotypically heterogenous tumor cells.

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Phenotypic heterogeneity in 3-methylcholanthrene - Induced transformation of normal rat kidney cells infected with Moloney murine leukemia virus

Abstract

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