Phloem-specific expression of the lectin gene from Allium sativum confers resistance to the sap-sucker Nilaparvata lugens

Kottakota Chandrasekhar, Muvva Vijayalakshmi, Kalasamudramu Vani, Tanushri Kaul, Malireddy K. Reddy

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Rice production is severely hampered by insect pests. Garlic lectin gene (ASAL) holds great promise in conferring protection against chewing (lepidopteran) and sap-sucking (homopteran) insect pests. We have developed transgenic rice lines resistant to sap-sucking brown hopper (Nilaparvata lugens) by ectopic expression of ASAL in their phloem tissues. Molecular analyses of T0 lines confirmed stable integration of transgene. T1 lines (NP 1-2, 4-3, 11-6 & 17-7) showed active transcription and translation of ASAL transgene. ELISA revealed ASAL expression was as high as 0.95 % of total soluble protein. Insect bioassays on T2 homozygous lines (NP 18 & 32) revealed significant reduction (~74-83 %) in survival rate, development and fecundity of brown hoppers in comparison to wild type. Transgenics exhibited enhanced resistance (1-2 score) against brown hoppers, minimal plant damage and no growth penalty or phenotypic abnormalities.

Original languageEnglish
Pages (from-to)1059-1067
Number of pages9
JournalBiotechnology Letters
Volume36
Issue number5
DOIs
StatePublished - 1 Jan 2014
Externally publishedYes

Keywords

  • Brown plant hopper
  • Garlic lectin
  • Phloem-specific promoter
  • Plant hopper insect
  • Rice
  • Sap sucker insect
  • Transgenic rice

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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