Phloem-specific expression of the lectin gene from Allium sativum confers resistance to the sap-sucker Nilaparvata lugens

Kottakota Chandrasekhar; Muvva Vijayalakshmi; Kalasamudramu Vani; Tanushri Kaul; Malireddy K. Reddy

doi:10.1007/s10529-014-1459-8

Phloem-specific expression of the lectin gene from Allium sativum confers resistance to the sap-sucker Nilaparvata lugens

Kottakota Chandrasekhar, Muvva Vijayalakshmi, Kalasamudramu Vani, Tanushri Kaul, Malireddy K. Reddy

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Rice production is severely hampered by insect pests. Garlic lectin gene (ASAL) holds great promise in conferring protection against chewing (lepidopteran) and sap-sucking (homopteran) insect pests. We have developed transgenic rice lines resistant to sap-sucking brown hopper (Nilaparvata lugens) by ectopic expression of ASAL in their phloem tissues. Molecular analyses of T₀ lines confirmed stable integration of transgene. T₁ lines (NP 1-2, 4-3, 11-6 & 17-7) showed active transcription and translation of ASAL transgene. ELISA revealed ASAL expression was as high as 0.95 % of total soluble protein. Insect bioassays on T₂ homozygous lines (NP 18 & 32) revealed significant reduction (~74-83 %) in survival rate, development and fecundity of brown hoppers in comparison to wild type. Transgenics exhibited enhanced resistance (1-2 score) against brown hoppers, minimal plant damage and no growth penalty or phenotypic abnormalities.

Original language	English
Pages (from-to)	1059-1067
Number of pages	9
Journal	Biotechnology Letters
Volume	36
Issue number	5
DOIs	https://doi.org/10.1007/s10529-014-1459-8
State	Published - 1 Jan 2014
Externally published	Yes

Keywords

Brown plant hopper
Garlic lectin
Phloem-specific promoter
Plant hopper insect
Rice
Sap sucker insect
Transgenic rice

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-014-1459-8

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title = "Phloem-specific expression of the lectin gene from Allium sativum confers resistance to the sap-sucker Nilaparvata lugens",

abstract = "Rice production is severely hampered by insect pests. Garlic lectin gene (ASAL) holds great promise in conferring protection against chewing (lepidopteran) and sap-sucking (homopteran) insect pests. We have developed transgenic rice lines resistant to sap-sucking brown hopper (Nilaparvata lugens) by ectopic expression of ASAL in their phloem tissues. Molecular analyses of T0 lines confirmed stable integration of transgene. T1 lines (NP 1-2, 4-3, 11-6 & 17-7) showed active transcription and translation of ASAL transgene. ELISA revealed ASAL expression was as high as 0.95 % of total soluble protein. Insect bioassays on T2 homozygous lines (NP 18 & 32) revealed significant reduction (~74-83 %) in survival rate, development and fecundity of brown hoppers in comparison to wild type. Transgenics exhibited enhanced resistance (1-2 score) against brown hoppers, minimal plant damage and no growth penalty or phenotypic abnormalities.",

keywords = "Brown plant hopper, Garlic lectin, Phloem-specific promoter, Plant hopper insect, Rice, Sap sucker insect, Transgenic rice",

author = "Kottakota Chandrasekhar and Muvva Vijayalakshmi and Kalasamudramu Vani and Tanushri Kaul and Reddy, {Malireddy K.}",

note = "Funding Information: Acknowledgments We are grateful to Department of Biotechnology, Government of India, New Delhi for the financial assistance. We thank Dr. Bentur, Directorate of Rice Research, Hyderabad for providing brown hoppers required for insect bioassays on transgenic plants.",

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doi = "10.1007/s10529-014-1459-8",

language = "English",

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pages = "1059--1067",

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T1 - Phloem-specific expression of the lectin gene from Allium sativum confers resistance to the sap-sucker Nilaparvata lugens

AU - Chandrasekhar, Kottakota

AU - Vijayalakshmi, Muvva

AU - Vani, Kalasamudramu

AU - Kaul, Tanushri

AU - Reddy, Malireddy K.

N1 - Funding Information: Acknowledgments We are grateful to Department of Biotechnology, Government of India, New Delhi for the financial assistance. We thank Dr. Bentur, Directorate of Rice Research, Hyderabad for providing brown hoppers required for insect bioassays on transgenic plants.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Rice production is severely hampered by insect pests. Garlic lectin gene (ASAL) holds great promise in conferring protection against chewing (lepidopteran) and sap-sucking (homopteran) insect pests. We have developed transgenic rice lines resistant to sap-sucking brown hopper (Nilaparvata lugens) by ectopic expression of ASAL in their phloem tissues. Molecular analyses of T0 lines confirmed stable integration of transgene. T1 lines (NP 1-2, 4-3, 11-6 & 17-7) showed active transcription and translation of ASAL transgene. ELISA revealed ASAL expression was as high as 0.95 % of total soluble protein. Insect bioassays on T2 homozygous lines (NP 18 & 32) revealed significant reduction (~74-83 %) in survival rate, development and fecundity of brown hoppers in comparison to wild type. Transgenics exhibited enhanced resistance (1-2 score) against brown hoppers, minimal plant damage and no growth penalty or phenotypic abnormalities.

AB - Rice production is severely hampered by insect pests. Garlic lectin gene (ASAL) holds great promise in conferring protection against chewing (lepidopteran) and sap-sucking (homopteran) insect pests. We have developed transgenic rice lines resistant to sap-sucking brown hopper (Nilaparvata lugens) by ectopic expression of ASAL in their phloem tissues. Molecular analyses of T0 lines confirmed stable integration of transgene. T1 lines (NP 1-2, 4-3, 11-6 & 17-7) showed active transcription and translation of ASAL transgene. ELISA revealed ASAL expression was as high as 0.95 % of total soluble protein. Insect bioassays on T2 homozygous lines (NP 18 & 32) revealed significant reduction (~74-83 %) in survival rate, development and fecundity of brown hoppers in comparison to wild type. Transgenics exhibited enhanced resistance (1-2 score) against brown hoppers, minimal plant damage and no growth penalty or phenotypic abnormalities.

KW - Brown plant hopper

KW - Garlic lectin

KW - Phloem-specific promoter

KW - Plant hopper insect

KW - Rice

KW - Sap sucker insect

KW - Transgenic rice

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C2 - 24563293

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VL - 36

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JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

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ER -

Phloem-specific expression of the lectin gene from Allium sativum confers resistance to the sap-sucker Nilaparvata lugens

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