Phorbol ester-induced lymphocyte adherence: Selective action on NK cells

S. Argov, M. Hebdon, P. Cuatrecasas, H. S. Koren

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Treatment of human peripheral blood lymphocytes (PBL) with phorbol dibutyrate (PDBU) for 20 to 45 min at 37°C induces adherence of 5 to 30% of the cells to plastic. The adherent cells (pAd) were highly enriched in NK cells on the basis of the following findings: 1) they exhibit high NK and ADCC activity but do not lyse the NK-resistant cell line, Daudi; 2) cytotoxic activity is enhanced by pretreatment with interferon-α (IFN-α); 3) the surface markers of these cells, as determined with monoclonal antibodies, are consistent with NK cells; and 4) they are enriched with cells morphologically similar to large granular lymphocytes. Conversely, the PDBU-nonadherent cells were substantially depleted of NK cells. The fact that the pAd cells do not lyse the Daudi line and that their NK activity can be further augmented by IFN-α would suggest that the pAd are enriched for NK cells rather than changed in their characteristics as a result of the separation procedure. Moreover, no consistent and appreciable modulation of NK activity induced by PDBU was observed. This report therefore demonstrates that PDBU selectively induces NK adherence of NK cells, which may have practical as well as biological implications.

Original languageEnglish
Pages (from-to)2215-2222
Number of pages8
JournalJournal of Immunology
Volume134
Issue number4
StatePublished - 1 Jan 1985
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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