Phospho-regulation of kinesin-5 during anaphase spindle elongation

Rachel Avunie-Masala, Natalia Movshovich, Yael Nissenkorn, Adina Gerson-Gurwitz, Vladimir Fridman, Mardo Kõivomägi, Mart Loog, M. Andrew Hoyt, Arieh Zaritsky, Larisa Gheber

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

The kinesin-5 Saccharomyces cerevisiae homologue Cin8 is shown here to be differentially phosphorylated during late anaphase at Cdk1-specific sites located in its motor domain. Wild-type Cin8 binds to the early-anaphase spindles and detaches from the spindles at late anaphase, whereas the phosphorylation-deficient Cin8-3A mutant protein remains attached to a larger region of the spindle and spindle poles for prolonged periods. This localization of Cin8-3A causes faster spindle elongation and longer anaphase spindles, which have aberrant morphology. By contrast, the phospho-mimic Cin8-3D mutant exhibits reduced binding to the spindles. In the absence of the kinesin-5 homologue Kip1, cells expressing Cin8-3D exhibit spindle assembly defects and are not viable at 37°C as a result of spindle collapse. We propose that dephosphorylation of Cin8 promotes its binding to the spindle microtubules before the onset of anaphase. In mid to late anaphase, phosphorylation of Cin8 causes its detachment from the spindles, which reduces the spindle elongation rate and aids in maintaining spindle morphology.

Original languageEnglish
Pages (from-to)873-878
Number of pages6
JournalJournal of Cell Science
Volume124
Issue number6
DOIs
StatePublished - 15 Mar 2011

Keywords

  • Cdk1
  • Cin8
  • Kinesin-5
  • Microtubules
  • Mitosis

ASJC Scopus subject areas

  • Cell Biology

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