Phosphonoformate inhibits synthesis of epstein-barr virus (EBV) capsid antigen and transformation of human cord blood lymphocytes by EBV

Miriam Margalith; Daphna Manor; Varda Usieli; Natan Goldblum

doi:10.1016/0042-6822(80)90086-0

Phosphonoformate inhibits synthesis of epstein-barr virus (EBV) capsid antigen and transformation of human cord blood lymphocytes by EBV

Miriam Margalith, Daphna Manor, Varda Usieli, Natan Goldblum

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Phosphonoformic acid trisodium salt (PF) and ethyl diethylphosphonoformate (Et-PF) inhibited Epstein-Barr virus (EBV) viral capsid antigen (VCA) synthesis in B.95-8 cells, at concentrations which were nontoxic to cells. PF inhibited 96.7% of VCA synthesis when used at 500 μM whereas Et-PF inhibited only 68.9% of VCA synthesis when used at 2000 μM. The synthesis of EBV nuclear antigen (EBNA) was not inhibited by PF or Et-PF. PF inhibited the transformation of human cord blood lymphocytes (CBL) by EBV as detected by [³H]thymidine uptake. The inhibition of transformation was a function of the dose of PF used. A concentration of 2000 μM PF completely inhibited the transformation; the same concentration of ET-PF had almost no effect.

Original language	English
Pages (from-to)	226-230
Number of pages	5
Journal	Virology
Volume	102
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(80)90086-0
State	Published - 15 Apr 1980
Externally published	Yes

ASJC Scopus subject areas

Virology

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10.1016/0042-6822(80)90086-0

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title = "Phosphonoformate inhibits synthesis of epstein-barr virus (EBV) capsid antigen and transformation of human cord blood lymphocytes by EBV",

abstract = "Phosphonoformic acid trisodium salt (PF) and ethyl diethylphosphonoformate (Et-PF) inhibited Epstein-Barr virus (EBV) viral capsid antigen (VCA) synthesis in B.95-8 cells, at concentrations which were nontoxic to cells. PF inhibited 96.7% of VCA synthesis when used at 500 μM whereas Et-PF inhibited only 68.9% of VCA synthesis when used at 2000 μM. The synthesis of EBV nuclear antigen (EBNA) was not inhibited by PF or Et-PF. PF inhibited the transformation of human cord blood lymphocytes (CBL) by EBV as detected by [3H]thymidine uptake. The inhibition of transformation was a function of the dose of PF used. A concentration of 2000 μM PF completely inhibited the transformation; the same concentration of ET-PF had almost no effect.",

author = "Miriam Margalith and Daphna Manor and Varda Usieli and Natan Goldblum",

note = "Funding Information: 1 This study was supported by grants from the Stiftung Volkswagen, the Max Bogen Research Fund, and the Judith Segal Medical Research Fund. * To whom reprint requests should be addressed. 3 M. Margalith, D. Manor, I. Agranat, Y. Bentor, T. Gelfand, and N. Goldblum, submitted for publication.",

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doi = "10.1016/0042-6822(80)90086-0",

language = "English",

volume = "102",

pages = "226--230",

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T1 - Phosphonoformate inhibits synthesis of epstein-barr virus (EBV) capsid antigen and transformation of human cord blood lymphocytes by EBV

AU - Margalith, Miriam

AU - Manor, Daphna

AU - Usieli, Varda

AU - Goldblum, Natan

N1 - Funding Information: 1 This study was supported by grants from the Stiftung Volkswagen, the Max Bogen Research Fund, and the Judith Segal Medical Research Fund. * To whom reprint requests should be addressed. 3 M. Margalith, D. Manor, I. Agranat, Y. Bentor, T. Gelfand, and N. Goldblum, submitted for publication.

PY - 1980/4/15

Y1 - 1980/4/15

N2 - Phosphonoformic acid trisodium salt (PF) and ethyl diethylphosphonoformate (Et-PF) inhibited Epstein-Barr virus (EBV) viral capsid antigen (VCA) synthesis in B.95-8 cells, at concentrations which were nontoxic to cells. PF inhibited 96.7% of VCA synthesis when used at 500 μM whereas Et-PF inhibited only 68.9% of VCA synthesis when used at 2000 μM. The synthesis of EBV nuclear antigen (EBNA) was not inhibited by PF or Et-PF. PF inhibited the transformation of human cord blood lymphocytes (CBL) by EBV as detected by [3H]thymidine uptake. The inhibition of transformation was a function of the dose of PF used. A concentration of 2000 μM PF completely inhibited the transformation; the same concentration of ET-PF had almost no effect.

AB - Phosphonoformic acid trisodium salt (PF) and ethyl diethylphosphonoformate (Et-PF) inhibited Epstein-Barr virus (EBV) viral capsid antigen (VCA) synthesis in B.95-8 cells, at concentrations which were nontoxic to cells. PF inhibited 96.7% of VCA synthesis when used at 500 μM whereas Et-PF inhibited only 68.9% of VCA synthesis when used at 2000 μM. The synthesis of EBV nuclear antigen (EBNA) was not inhibited by PF or Et-PF. PF inhibited the transformation of human cord blood lymphocytes (CBL) by EBV as detected by [3H]thymidine uptake. The inhibition of transformation was a function of the dose of PF used. A concentration of 2000 μM PF completely inhibited the transformation; the same concentration of ET-PF had almost no effect.

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ER -

Phosphonoformate inhibits synthesis of epstein-barr virus (EBV) capsid antigen and transformation of human cord blood lymphocytes by EBV

Abstract

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