TY - JOUR
T1 - Phosphorothioate Oligodeoxynucleotides Are Potent Sequence Nonspecific Inhibitors of De Novo Infection by HIV
AU - Stein, C. A.
AU - Matsukura, Makoto
AU - Subasinghe, Chrisanthe
AU - Broder, Samuel
AU - Cohen, Jack S.
PY - 1989/1/1
Y1 - 1989/1/1
N2 - Phosphorothioate homo-oligodeoxynucleotides have recently been found to protect ATH-8 cells against the cytopathic effect of de novo infection by HIV. The effect is dose and chain-length dependent, with a maximum effect seen for 21–28-mers. We have now synthesized a series of phosphorothioate oligomers with mixed-based sequences and found that all of them have a dose-dependent cytoprotective effect that is maximal at an oligomer concentration of about 1–2 μM. The least effective sequences contain only A or T, and the most effective sequences have 40% GC content or greater. The results also confirm the length effect, namely that 21-mers are more cytoprotective than 14-mers.
AB - Phosphorothioate homo-oligodeoxynucleotides have recently been found to protect ATH-8 cells against the cytopathic effect of de novo infection by HIV. The effect is dose and chain-length dependent, with a maximum effect seen for 21–28-mers. We have now synthesized a series of phosphorothioate oligomers with mixed-based sequences and found that all of them have a dose-dependent cytoprotective effect that is maximal at an oligomer concentration of about 1–2 μM. The least effective sequences contain only A or T, and the most effective sequences have 40% GC content or greater. The results also confirm the length effect, namely that 21-mers are more cytoprotective than 14-mers.
UR - http://www.scopus.com/inward/record.url?scp=0024814327&partnerID=8YFLogxK
U2 - 10.1089/aid.1989.5.639
DO - 10.1089/aid.1989.5.639
M3 - Article
C2 - 2482061
AN - SCOPUS:0024814327
SN - 0889-2229
VL - 5
SP - 639
EP - 646
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
IS - 6
ER -
