Phosphorus-31 relaxation times of inorganic phosphate and nucleotides in aqueous solution

J. Granot; G. A. Elgavish; J. S. Cohen

doi:10.1016/0022-2364(79)90168-9

Phosphorus-31 relaxation times of inorganic phosphate and nucleotides in aqueous solution

J. Granot, G. A. Elgavish, J. S. Cohen

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Abstract

³¹P spin-lattice relaxation times (T₁) of inorganic orthophosphate, and of the nucleotides TMP, AMP and ATP are reported as a function of pH. The T₁ values are found to exhibit significant pH dependences, with minimum T₁ values generally observed at pH values close to the pK_a values for the orthophosphates. The effects of the addition of Cu²⁺ or EDTA and treatment with cation exchange resin are described. These observations are related to similar results obtained previously for other ionizing species using ¹H and ¹³C NMR, indicating a general phenomenon of such pH-dependent variations in T₁ values in aqueous solutions. It is suggested that this effect stems from interactions with paramagnetic metal ion impurities.

Original language	English
Pages (from-to)	569-575
Number of pages	7
Journal	Journal of Magnetic Resonance (1969)
Volume	33
Issue number	3
DOIs	https://doi.org/10.1016/0022-2364(79)90168-9
State	Published - 1 Jan 1979
Externally published	Yes

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title = "Phosphorus-31 relaxation times of inorganic phosphate and nucleotides in aqueous solution",

abstract = "31P spin-lattice relaxation times (T1) of inorganic orthophosphate, and of the nucleotides TMP, AMP and ATP are reported as a function of pH. The T1 values are found to exhibit significant pH dependences, with minimum T1 values generally observed at pH values close to the pKa values for the orthophosphates. The effects of the addition of Cu2+ or EDTA and treatment with cation exchange resin are described. These observations are related to similar results obtained previously for other ionizing species using 1H and 13C NMR, indicating a general phenomenon of such pH-dependent variations in T1 values in aqueous solutions. It is suggested that this effect stems from interactions with paramagnetic metal ion impurities.",

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T1 - Phosphorus-31 relaxation times of inorganic phosphate and nucleotides in aqueous solution

AU - Granot, J.

AU - Elgavish, G. A.

AU - Cohen, J. S.

PY - 1979/1/1

Y1 - 1979/1/1

N2 - 31P spin-lattice relaxation times (T1) of inorganic orthophosphate, and of the nucleotides TMP, AMP and ATP are reported as a function of pH. The T1 values are found to exhibit significant pH dependences, with minimum T1 values generally observed at pH values close to the pKa values for the orthophosphates. The effects of the addition of Cu2+ or EDTA and treatment with cation exchange resin are described. These observations are related to similar results obtained previously for other ionizing species using 1H and 13C NMR, indicating a general phenomenon of such pH-dependent variations in T1 values in aqueous solutions. It is suggested that this effect stems from interactions with paramagnetic metal ion impurities.

AB - 31P spin-lattice relaxation times (T1) of inorganic orthophosphate, and of the nucleotides TMP, AMP and ATP are reported as a function of pH. The T1 values are found to exhibit significant pH dependences, with minimum T1 values generally observed at pH values close to the pKa values for the orthophosphates. The effects of the addition of Cu2+ or EDTA and treatment with cation exchange resin are described. These observations are related to similar results obtained previously for other ionizing species using 1H and 13C NMR, indicating a general phenomenon of such pH-dependent variations in T1 values in aqueous solutions. It is suggested that this effect stems from interactions with paramagnetic metal ion impurities.

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DO - 10.1016/0022-2364(79)90168-9

M3 - Article

AN - SCOPUS:2142648872

SN - 0022-2364

VL - 33

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EP - 575

JO - Journal of Magnetic Resonance (1969)

JF - Journal of Magnetic Resonance (1969)

IS - 3

ER -

Phosphorus-31 relaxation times of inorganic phosphate and nucleotides in aqueous solution

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