Phosphorylation of Nucleotides Bound to Chloroplast Membranes and Their Role in Photophosphorylation

C Aflafo, N Shavit

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

1. The initial rapid phosphorylation of membrane-bound ADP yields [γ-32P]-ATP. Long term illumination of chloroplasts resulted in the introduction of label in the β position of ADP and ATP.
2. It is concluded that the initial acceptor in photophosphorylation is ADP and not AMP. The appearance of 32Pi in the β position of the nucleotide fractions is probably the result of side reactions not directly involved in the photophosphorylation mechanism.
3. The inhibitor phlorizin affects similarly the phosphorylation of bound ADP and the net photophosphorylation reaction.
4. The uncoupler nigericin has different effects on the phosphorylation of membrane-bound ADP and the net photophosphorylation reaction. Dissipation of energy by low concentrations of the uncoupler affects primarily the step(s) of ATP release to the medium, while steps leading to the interconversion of Pi and ADP to form bound ATP are less sensitive and are inhibited by relatively higher uncoupler concentrations.
Original languageEnglish
Pages (from-to)522-530
Number of pages9
JournalBiochimica et Biophysica Acta - General Subjects
Volume440
Issue number3
DOIs
StatePublished - 1976

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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