Phosphorylation of purified mitochondrial Voltage-Dependent Anion Channel by c-Jun N-terminal Kinase-3 modifies channel voltage-dependence

Rajeev Gupta, Subhendu Ghosh

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Voltage-Dependent Anion Channel (VDAC) phosphorylated by c-Jun N-terminal Kinase-3 (JNK3) was incorporated into the bilayer lipid membrane. Single-channel electrophysiological properties of the native and the phosphorylated VDAC were compared. The open probability versus voltage curve of the native VDAC displayed symmetry around the voltage axis, whereas that of the phosphorylated VDAC showed asymmetry. This result indicates that phosphorylation by JNK3 modifies voltage-dependence of VDAC.

Original languageEnglish
Pages (from-to)78-87
Number of pages10
JournalBiochimie Open
Volume4
DOIs
StatePublished - 1 Jun 2017
Externally publishedYes

Keywords

  • Bilayer electrophysiology
  • Phosphorylation
  • Voltage-Dependent Anion Channel
  • c-Jun N-terminal Kinase-3

ASJC Scopus subject areas

  • Biochemistry

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