Photochrome-labeling method in study of dynamics of biological systems

G. I. Likhtenshtein; D. V. Khudjakov; V. R. Vogel

doi:10.1016/0165-022X(92)90016-4

Photochrome-labeling method in study of dynamics of biological systems

G. I. Likhtenshtein, D. V. Khudjakov, V. R. Vogel

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The theoretical considerations and experimental evidences discussed in this paper indicate that quantitative study of photochromic processes in labeled objects open up new possibilities for investigating microviscosity and conformation transitions in biological systems. The proposed method of photochrome labeling features higher sensitivity and simplicity, and uses labels which are more stable under physiological conditions compared with traditional spin labels.

Original language	English
Pages (from-to)	219-229
Number of pages	11
Journal	Journal of Biochemical and Biophysical Methods
Volume	25
Issue number	4
DOIs	https://doi.org/10.1016/0165-022X(92)90016-4
State	Published - 1 Jan 1992
Externally published	Yes

Keywords

Microviscosity
Myoglobin
Photochrome label
Protein dynamics

ASJC Scopus subject areas

Biophysics
Biochemistry

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10.1016/0165-022X(92)90016-4

Cite this

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title = "Photochrome-labeling method in study of dynamics of biological systems",

abstract = "The theoretical considerations and experimental evidences discussed in this paper indicate that quantitative study of photochromic processes in labeled objects open up new possibilities for investigating microviscosity and conformation transitions in biological systems. The proposed method of photochrome labeling features higher sensitivity and simplicity, and uses labels which are more stable under physiological conditions compared with traditional spin labels.",

keywords = "Microviscosity, Myoglobin, Photochrome label, Protein dynamics",

author = "Likhtenshtein, {G. I.} and Khudjakov, {D. V.} and Vogel, {V. R.}",

year = "1992",

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T1 - Photochrome-labeling method in study of dynamics of biological systems

AU - Likhtenshtein, G. I.

AU - Khudjakov, D. V.

AU - Vogel, V. R.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - The theoretical considerations and experimental evidences discussed in this paper indicate that quantitative study of photochromic processes in labeled objects open up new possibilities for investigating microviscosity and conformation transitions in biological systems. The proposed method of photochrome labeling features higher sensitivity and simplicity, and uses labels which are more stable under physiological conditions compared with traditional spin labels.

AB - The theoretical considerations and experimental evidences discussed in this paper indicate that quantitative study of photochromic processes in labeled objects open up new possibilities for investigating microviscosity and conformation transitions in biological systems. The proposed method of photochrome labeling features higher sensitivity and simplicity, and uses labels which are more stable under physiological conditions compared with traditional spin labels.

KW - Microviscosity

KW - Myoglobin

KW - Photochrome label

KW - Protein dynamics

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U2 - 10.1016/0165-022X(92)90016-4

DO - 10.1016/0165-022X(92)90016-4

M3 - Article

AN - SCOPUS:0026454960

SN - 0165-022X

VL - 25

SP - 219

EP - 229

JO - Journal of Biochemical and Biophysical Methods

JF - Journal of Biochemical and Biophysical Methods

IS - 4

ER -

Photochrome-labeling method in study of dynamics of biological systems

Abstract

Keywords

ASJC Scopus subject areas

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