Photocontrol of tyrosine phosphorylation in mammalian cells via genetic encoding of photocaged tyrosine

Eyal Arbely; Jessica Torres-Kolbus; Alexander Deiters; Jason W. Chin

doi:10.1021/ja3046958

Photocontrol of tyrosine phosphorylation in mammalian cells via genetic encoding of photocaged tyrosine

Eyal Arbely, Jessica Torres-Kolbus, Alexander Deiters, Jason W. Chin

Research output: Contribution to journal › Article › peer-review

140 Scopus citations

Abstract

We report the first site-specific genetic encoding of photocaged tyrosine into proteins in mammalian cells. By photocaging Tyr701 of STAT1 we demonstrate that it is possible to photocontrol tyrosine phosphorylation and signal transduction in mammalian cells.

Original language	English
Pages (from-to)	11912-11915
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	134
Issue number	29
DOIs	https://doi.org/10.1021/ja3046958
State	Published - 25 Jul 2012
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja3046958

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title = "Photocontrol of tyrosine phosphorylation in mammalian cells via genetic encoding of photocaged tyrosine",

abstract = "We report the first site-specific genetic encoding of photocaged tyrosine into proteins in mammalian cells. By photocaging Tyr701 of STAT1 we demonstrate that it is possible to photocontrol tyrosine phosphorylation and signal transduction in mammalian cells.",

author = "Eyal Arbely and Jessica Torres-Kolbus and Alexander Deiters and Chin, {Jason W.}",

year = "2012",

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doi = "10.1021/ja3046958",

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AU - Arbely, Eyal

AU - Torres-Kolbus, Jessica

AU - Deiters, Alexander

AU - Chin, Jason W.

PY - 2012/7/25

Y1 - 2012/7/25

N2 - We report the first site-specific genetic encoding of photocaged tyrosine into proteins in mammalian cells. By photocaging Tyr701 of STAT1 we demonstrate that it is possible to photocontrol tyrosine phosphorylation and signal transduction in mammalian cells.

AB - We report the first site-specific genetic encoding of photocaged tyrosine into proteins in mammalian cells. By photocaging Tyr701 of STAT1 we demonstrate that it is possible to photocontrol tyrosine phosphorylation and signal transduction in mammalian cells.

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DO - 10.1021/ja3046958

M3 - Article

AN - SCOPUS:84864276237

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 29

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Photocontrol of tyrosine phosphorylation in mammalian cells via genetic encoding of photocaged tyrosine

Abstract

ASJC Scopus subject areas

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