Photon-Counting Fluorescence Imaging of Tobacco Cultured Cells Through Scattering Medium Using Transport of Intensity Equation and Iterative Phase Retrieval Method

Shiori Matsuda, Naru Yoneda, Manoj Kumar, Osamu Matoba

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this paper, we combine photon-counting imaging and the transport of intensity equation (TIE) fluorescent imaging through a scattering medium to present three-dimensional fluorescent imaging under weak light conditions. Photon-counting fluorescent images of tobacco-cultured cells through a diffuser are recorded and the complex amplitude distribution is obtained using TIE. The iterative phase retrieval method is then applied to remove residual blurred noise.

Original languageEnglish
Title of host publication16th Pacific Rim Conference on Lasers and Electro-Optics, CLEO-PR 2024
PublisherInstitute of Electrical and Electronics Engineers
ISBN (Electronic)9798350372076
DOIs
StatePublished - 1 Jan 2024
Externally publishedYes
Event16th Pacific Rim Conference on Lasers and Electro-Optics, CLEO-PR 2024 - Incheon, Korea, Republic of
Duration: 4 Aug 20249 Aug 2024

Publication series

Name16th Pacific Rim Conference on Lasers and Electro-Optics, CLEO-PR 2024

Conference

Conference16th Pacific Rim Conference on Lasers and Electro-Optics, CLEO-PR 2024
Country/TerritoryKorea, Republic of
CityIncheon
Period4/08/249/08/24

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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