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Photon-counting fluorescence imaging of tobacco cultured cells through scattering medium using transport of intensity equation and iterative phase retrieval method

  • Shiori Matsuda
  • , Naru Yoneda
  • , Manoj Kumar
  • , Osamu Matoba

Research output: Contribution to conferencePaperpeer-review

Abstract

In this paper, we combine photon-counting imaging and the transport of intensity equation (TIE) fluorescent imaging through a scattering medium to present three-dimensional fluorescent imaging under weak light conditions. Photon-counting fluorescent images of tobacco-cultured cells through a diffuser are recorded and the complex amplitude distribution is obtained using TIE. The iterative phase retrieval method is then applied to remove residual blurred noise.

Original languageEnglish
StatePublished - 1 Jan 2024
Externally publishedYes
Event2024 Conference on Lasers and Electro-Optics/Pacific Rim, CLEO-PR 2024 - Incheon, Korea, Republic of
Duration: 4 Aug 20248 Aug 2024

Conference

Conference2024 Conference on Lasers and Electro-Optics/Pacific Rim, CLEO-PR 2024
Country/TerritoryKorea, Republic of
CityIncheon
Period4/08/248/08/24

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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