The present invention provides a photoreactive reagent that binds specifical ly to Ca2+-binding proteins, links to them covalently after photo-activation, a nd labels them. The novel reagent enables the characterization, purification, inhibition and screening of Ca2+-binding proteins, as well as the preparatio n of a new affinity chromatography matrix and a new protein biosensor. The invention also relates to the use of the reagent in inhibiting apoptosis and necrosis and in diagnosing a disorder associated with a defect in the functi on of a Ca2+-binding protein, and in the preparation of a medicament for treati ng such disorders.
|IPC||C12N 11/ 14 A I|
|State||Published - 1 Sep 2005|