TY - JOUR
T1 - Placental secretion of interleukin-1 and interleukin-1 receptor antagonist in preeclampsia
T2 - Effect of magnesium sulfate
AU - Amash, Alaa
AU - Holcberg, Gershon
AU - Sapir, Olga
AU - Huleihel, Mahmoud
PY - 2012/9/1
Y1 - 2012/9/1
N2 - Preeclampsia is a pregnancy-specific disorder characterized by hypertension and systemic endothelial dysfunction. Interleukin (IL)-1β is a possible mediator of maternal endothelial dysfunction in preeclampsia. Serum IL-1β as well as its natural inhibitor IL-1 receptor antagonist (IL-1Ra) were reported to be increased in women with preeclampsia. In the current study, we addressed the role of the placenta in controlling the circulatory levels of IL-1β and its natural inhibitor IL-1Ra in preeclampsia, and the possible effect of magnesium sulfate (MgSO4) on these levels. Using an ex vivo placental perfusion system, placentas from preeclamptic (n=9) and normotensive (n=6) pregnancies were perfused in presence or absence of MgSO4. Perfusate samples were collected from the maternal and the fetal circulations of the perfusion system, and IL-1β and IL-1Ra were examined by enzyme-linked immunoassay (ELISA). Preeclamptic placentas secreted higher levels of IL-1β (P<0.001), and a tendentious higher levels of IL-1Ra, mainly into the maternal circulation, as compared with normotensive placentas, although no differences in IL-1β:IL-1Ra ratio were detected. However, there was only tendentious increase in the secretion levels of IL-1β or IL-1Ra into the fetal circulation of preeclamptic placentas, when compared with normotensive placentas. Administration of MgSO4 to preeclamptic placentas resulted in an attenuation of the increased secretion of IL-1β into the maternal circulation (P<0.001), and in a tendentious reduction in IL-1Ra. However, IL-1β:IL-1Ra ratio in preeclamptic placentas was not affected by MgSO 4. Interestingly, exposure of normotensive placenta to MgSO 4 resulted only in increased levels of IL-1Ra in the maternal circulation, without affecting IL-1β levels or IL-1β:IL-1Ra ratio. These findings suggest that the placenta may contribute to the elevation in serum IL-1β and IL-1Ra in preeclampsia by increased secretion of these cytokines into the maternal circulation, and that MgSO4 is able to attenuate this increased secretion of IL-1β, and possibly IL-1Ra, in preeclampsia.
AB - Preeclampsia is a pregnancy-specific disorder characterized by hypertension and systemic endothelial dysfunction. Interleukin (IL)-1β is a possible mediator of maternal endothelial dysfunction in preeclampsia. Serum IL-1β as well as its natural inhibitor IL-1 receptor antagonist (IL-1Ra) were reported to be increased in women with preeclampsia. In the current study, we addressed the role of the placenta in controlling the circulatory levels of IL-1β and its natural inhibitor IL-1Ra in preeclampsia, and the possible effect of magnesium sulfate (MgSO4) on these levels. Using an ex vivo placental perfusion system, placentas from preeclamptic (n=9) and normotensive (n=6) pregnancies were perfused in presence or absence of MgSO4. Perfusate samples were collected from the maternal and the fetal circulations of the perfusion system, and IL-1β and IL-1Ra were examined by enzyme-linked immunoassay (ELISA). Preeclamptic placentas secreted higher levels of IL-1β (P<0.001), and a tendentious higher levels of IL-1Ra, mainly into the maternal circulation, as compared with normotensive placentas, although no differences in IL-1β:IL-1Ra ratio were detected. However, there was only tendentious increase in the secretion levels of IL-1β or IL-1Ra into the fetal circulation of preeclamptic placentas, when compared with normotensive placentas. Administration of MgSO4 to preeclamptic placentas resulted in an attenuation of the increased secretion of IL-1β into the maternal circulation (P<0.001), and in a tendentious reduction in IL-1Ra. However, IL-1β:IL-1Ra ratio in preeclamptic placentas was not affected by MgSO 4. Interestingly, exposure of normotensive placenta to MgSO 4 resulted only in increased levels of IL-1Ra in the maternal circulation, without affecting IL-1β levels or IL-1β:IL-1Ra ratio. These findings suggest that the placenta may contribute to the elevation in serum IL-1β and IL-1Ra in preeclampsia by increased secretion of these cytokines into the maternal circulation, and that MgSO4 is able to attenuate this increased secretion of IL-1β, and possibly IL-1Ra, in preeclampsia.
UR - http://www.scopus.com/inward/record.url?scp=84866278626&partnerID=8YFLogxK
U2 - 10.1089/jir.2012.0013
DO - 10.1089/jir.2012.0013
M3 - Article
C2 - 22909148
AN - SCOPUS:84866278626
SN - 1079-9907
VL - 32
SP - 432
EP - 441
JO - Journal of Interferon and Cytokine Research
JF - Journal of Interferon and Cytokine Research
IS - 9
ER -