Plant cell observation by TIE-based fluorescence imaging

Sudheesh K. Rajput; Osamu Matoba; Manoj Kumar; Xiangyu Quan; Yasuhiro Awatsuji; Yosuke Tamada

Plant cell observation by TIE-based fluorescence imaging

Sudheesh K. Rajput, Osamu Matoba, Manoj Kumar, Xiangyu Quan, Yasuhiro Awatsuji, Yosuke Tamada

Research output: Contribution to journal › Conference article › peer-review

Abstract

We present fluorescence imaging of plant cells based on transport of intensity equation and Fresnel propagation. At first, the phase distribution is obtained from the recorded three-defocus fluorescence intensity images and then from phase and corresponding intensity defocus image, the focus images of plant cell sample can be retrieved at various planes after free space propagation.

Original language	English
Article number	DTh4A.3
Journal	Optics InfoBase Conference Papers
State	Published - 1 Jan 2020
Externally published	Yes
Event	3D Image Acquisition and Display: Technology, Perception and Applications, 3D 2020 - Part of Imaging and Applied Optics Congress - Virtual, Online, United States Duration: 22 Jun 2020 → 26 Jun 2020

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Mechanics of Materials

Cite this

@article{2e27a5ef0caa4310bcc96771db66217f,

title = "Plant cell observation by TIE-based fluorescence imaging",

abstract = "We present fluorescence imaging of plant cells based on transport of intensity equation and Fresnel propagation. At first, the phase distribution is obtained from the recorded three-defocus fluorescence intensity images and then from phase and corresponding intensity defocus image, the focus images of plant cell sample can be retrieved at various planes after free space propagation.",

author = "Rajput, {Sudheesh K.} and Osamu Matoba and Manoj Kumar and Xiangyu Quan and Yasuhiro Awatsuji and Yosuke Tamada",

note = "Publisher Copyright: {\textcopyright} OSA 2020; 3D Image Acquisition and Display: Technology, Perception and Applications, 3D 2020 - Part of Imaging and Applied Optics Congress ; Conference date: 22-06-2020 Through 26-06-2020",

year = "2020",

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language = "English",

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issn = "2162-2701",

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TY - JOUR

T1 - Plant cell observation by TIE-based fluorescence imaging

AU - Rajput, Sudheesh K.

AU - Matoba, Osamu

AU - Kumar, Manoj

AU - Quan, Xiangyu

AU - Awatsuji, Yasuhiro

AU - Tamada, Yosuke

PY - 2020/1/1

Y1 - 2020/1/1

N2 - We present fluorescence imaging of plant cells based on transport of intensity equation and Fresnel propagation. At first, the phase distribution is obtained from the recorded three-defocus fluorescence intensity images and then from phase and corresponding intensity defocus image, the focus images of plant cell sample can be retrieved at various planes after free space propagation.

AB - We present fluorescence imaging of plant cells based on transport of intensity equation and Fresnel propagation. At first, the phase distribution is obtained from the recorded three-defocus fluorescence intensity images and then from phase and corresponding intensity defocus image, the focus images of plant cell sample can be retrieved at various planes after free space propagation.

UR - http://www.scopus.com/inward/record.url?scp=85118605098&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:85118605098

SN - 2162-2701

JO - Optics InfoBase Conference Papers

JF - Optics InfoBase Conference Papers

M1 - DTh4A.3

T2 - 3D Image Acquisition and Display: Technology, Perception and Applications, 3D 2020 - Part of Imaging and Applied Optics Congress

Y2 - 22 June 2020 through 26 June 2020

ER -

Plant cell observation by TIE-based fluorescence imaging

Abstract

ASJC Scopus subject areas

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