TY - JOUR
T1 - Plasma extracellular vesicles enriched for neuronal origin
T2 - A potential window into brain pathologic processes
AU - Mustapic, Maja
AU - Eitan, Erez
AU - Werner, John K.
AU - Berkowitz, Sean T.
AU - Lazaropoulos, Michael P.
AU - Tran, Joyce
AU - Goetzl, Edward J.
AU - Kapogiannis, Dimitrios
N1 - Publisher Copyright:
© 2017 Mustapic, Eitan, Werner, Berkowitz, Lazaropoulos, Tran, Goetzl and Kapogiannis.
PY - 2017/5/22
Y1 - 2017/5/22
N2 - Our team has been a pioneer in harvesting extracellular vesicles (EVs) enriched for neuronal origin from peripheral blood and using them as a biomarker discovery platform for neurological disorders. This methodology has demonstrated excellent diagnostic and predictive performance for Alzheimer's and other neurodegenerative diseases in multiple studies, providing a strong proof of concept for this approach. Here, we describe our methodology in detail and offer further evidence that isolated EVs are enriched for neuronal origin. In addition, we present evidence that EVs enriched for neuronal origin represent a more sensitive and accurate base for biomarkers than plasma, serum, or non-enriched total plasma EVs. Finally, we proceed to investigate the protein content of EVs enriched for neuronal origin and compare it with other relevant enriched and non-enriched populations of plasma EVs. Neuronal-origin enriched plasma EVs contain higher levels of signaling molecules of great interest for cellular metabolism, survival, and repair, which may be useful as biomarkers and to follow response to therapeutic interventions in a mechanism-specific manner.
AB - Our team has been a pioneer in harvesting extracellular vesicles (EVs) enriched for neuronal origin from peripheral blood and using them as a biomarker discovery platform for neurological disorders. This methodology has demonstrated excellent diagnostic and predictive performance for Alzheimer's and other neurodegenerative diseases in multiple studies, providing a strong proof of concept for this approach. Here, we describe our methodology in detail and offer further evidence that isolated EVs are enriched for neuronal origin. In addition, we present evidence that EVs enriched for neuronal origin represent a more sensitive and accurate base for biomarkers than plasma, serum, or non-enriched total plasma EVs. Finally, we proceed to investigate the protein content of EVs enriched for neuronal origin and compare it with other relevant enriched and non-enriched populations of plasma EVs. Neuronal-origin enriched plasma EVs contain higher levels of signaling molecules of great interest for cellular metabolism, survival, and repair, which may be useful as biomarkers and to follow response to therapeutic interventions in a mechanism-specific manner.
KW - Alzheimer's disease
KW - Biological markers
KW - Extracellular vesicles (EVs)
KW - Liquid biopsy diagnostics
KW - Phosphorylated tau protein
UR - https://www.scopus.com/pages/publications/85019605047
U2 - 10.3389/fnins.2017.00278
DO - 10.3389/fnins.2017.00278
M3 - Article
AN - SCOPUS:85019605047
SN - 1662-4548
VL - 11
JO - Frontiers in Neuroscience
JF - Frontiers in Neuroscience
IS - MAY
M1 - 278
ER -