Plasmodium falciparum: In vitro induction of resistance to aminopterin

J. Golenser; D. Casuto; Y. Pollack

doi:10.1016/0014-4894(81)90095-3

Plasmodium falciparum: In vitro induction of resistance to aminopterin

J. Golenser, D. Casuto, Y. Pollack

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Plasmodium falciparum parasites were grown on microplates in the presence of aminopterin. The FCR-8 strain was more sensitive to aminopterin than a Richards strain and died within 1 week of treatment. A few parasites of the Richards strain survived treatment and developed normal parasitemias. This strain was resistant to aminopterin at concentrations not higher than those used for its selection. Removal of aminopterin did not affect the growth of the resistant variant, showing that it was not aminopterin dependent. Aminopterin affected the sensitive parasites by interfering with nucleic acid synthesis, whereas protein synthesis was not impaired. Gametocytogenesis was unaffected by aminopterin.

Original language	English
Pages (from-to)	371-377
Number of pages	7
Journal	Experimental Parasitology
Volume	52
Issue number	3
DOIs	https://doi.org/10.1016/0014-4894(81)90095-3
State	Published - 1 Jan 1981
Externally published	Yes

Keywords

Aminopterin
Culture, in vitro
Drug resistance
Hypoxanthine incorporation
Isoleucine incorporation
Malaria
Plasmodium falciparum
Primate
Protozoa, parasitic

ASJC Scopus subject areas

Parasitology
Immunology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0014-4894(81)90095-3

Cite this

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title = "Plasmodium falciparum: In vitro induction of resistance to aminopterin",

abstract = "Plasmodium falciparum parasites were grown on microplates in the presence of aminopterin. The FCR-8 strain was more sensitive to aminopterin than a Richards strain and died within 1 week of treatment. A few parasites of the Richards strain survived treatment and developed normal parasitemias. This strain was resistant to aminopterin at concentrations not higher than those used for its selection. Removal of aminopterin did not affect the growth of the resistant variant, showing that it was not aminopterin dependent. Aminopterin affected the sensitive parasites by interfering with nucleic acid synthesis, whereas protein synthesis was not impaired. Gametocytogenesis was unaffected by aminopterin.",

keywords = "Aminopterin, Culture, in vitro, Drug resistance, Hypoxanthine incorporation, Isoleucine incorporation, Malaria, Plasmodium falciparum, Primate, Protozoa, parasitic",

author = "J. Golenser and D. Casuto and Y. Pollack",

note = "Funding Information: ACKNOWLEDGMENTS This investigation received financial support from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases, and from the S. F. Kurvin Centre for the Study of Infectious and Tropical Diseases.",

year = "1981",

month = jan,

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doi = "10.1016/0014-4894(81)90095-3",

language = "English",

volume = "52",

pages = "371--377",

journal = "Experimental Parasitology",

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T1 - Plasmodium falciparum

T2 - In vitro induction of resistance to aminopterin

AU - Golenser, J.

AU - Casuto, D.

AU - Pollack, Y.

N1 - Funding Information: ACKNOWLEDGMENTS This investigation received financial support from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases, and from the S. F. Kurvin Centre for the Study of Infectious and Tropical Diseases.

PY - 1981/1/1

Y1 - 1981/1/1

N2 - Plasmodium falciparum parasites were grown on microplates in the presence of aminopterin. The FCR-8 strain was more sensitive to aminopterin than a Richards strain and died within 1 week of treatment. A few parasites of the Richards strain survived treatment and developed normal parasitemias. This strain was resistant to aminopterin at concentrations not higher than those used for its selection. Removal of aminopterin did not affect the growth of the resistant variant, showing that it was not aminopterin dependent. Aminopterin affected the sensitive parasites by interfering with nucleic acid synthesis, whereas protein synthesis was not impaired. Gametocytogenesis was unaffected by aminopterin.

AB - Plasmodium falciparum parasites were grown on microplates in the presence of aminopterin. The FCR-8 strain was more sensitive to aminopterin than a Richards strain and died within 1 week of treatment. A few parasites of the Richards strain survived treatment and developed normal parasitemias. This strain was resistant to aminopterin at concentrations not higher than those used for its selection. Removal of aminopterin did not affect the growth of the resistant variant, showing that it was not aminopterin dependent. Aminopterin affected the sensitive parasites by interfering with nucleic acid synthesis, whereas protein synthesis was not impaired. Gametocytogenesis was unaffected by aminopterin.

KW - Aminopterin

KW - Culture, in vitro

KW - Drug resistance

KW - Hypoxanthine incorporation

KW - Isoleucine incorporation

KW - Malaria

KW - Plasmodium falciparum

KW - Primate

KW - Protozoa, parasitic

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U2 - 10.1016/0014-4894(81)90095-3

DO - 10.1016/0014-4894(81)90095-3

M3 - Article

AN - SCOPUS:0019821777

SN - 0014-4894

VL - 52

SP - 371

EP - 377

JO - Experimental Parasitology

JF - Experimental Parasitology

IS - 3

ER -

Plasmodium falciparum: In vitro induction of resistance to aminopterin

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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