Plasmonic nanoprobes for stimulated emission depletion microscopy

Emiliano Cortes; Paloma A. Huidobro; Hugo G. Sinclair; Stina Guldbrand; William J. Peveler; Timothy Davies; Simona Parrinello; Frederik Görlitz; Chris Dunsby; Mark A. A. Neil; Yonatan Sivan; Ivan P. Parkin; Paul M. French; Stefan A. Maier

Plasmonic nanoprobes for stimulated emission depletion microscopy

Emiliano Cortes, Paloma A. Huidobro, Hugo G. Sinclair, Stina Guldbrand, William J. Peveler, Timothy Davies, Simona Parrinello, Frederik Görlitz, Chris Dunsby, Mark A. A. Neil, Yonatan Sivan, Ivan P. Parkin, Paul M. French, Stefan A. Maier

Unit of Photonics and Electro-Optics Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles in order to enhance the stimulated depletion of excited molecules for super-resolved microscopy. We demonstrate stimulated emission depletion (STED) microscopy with gold nanorods with a long axis of only 26 nm and a width of 8 nm that provide an enhancement of the resolution compared to fluorescent-only probes without plasmonic components irradiated with the same depletion power. These novel nanoparticle-assisted STED probes represent a ~2x10^3 reduction in probe volume compared to previously used nanoparticles and we demonstrate their application to the first plasmon-assisted STED cellular imaging. We also discuss their current limitations.Comment: 30 pages, 15 figures, main text and supplementary informatio

Original language	English GB
Journal	ACS Nano
State	Published - 5 Oct 2016

Keywords

Physics - Optics
Physics - Chemical Physics

Cite this

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title = "Plasmonic nanoprobes for stimulated emission depletion microscopy",

abstract = "Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles in order to enhance the stimulated depletion of excited molecules for super-resolved microscopy. We demonstrate stimulated emission depletion (STED) microscopy with gold nanorods with a long axis of only 26 nm and a width of 8 nm that provide an enhancement of the resolution compared to fluorescent-only probes without plasmonic components irradiated with the same depletion power. These novel nanoparticle-assisted STED probes represent a ~2x10^3 reduction in probe volume compared to previously used nanoparticles and we demonstrate their application to the first plasmon-assisted STED cellular imaging. We also discuss their current limitations.Comment: 30 pages, 15 figures, main text and supplementary informatio",

keywords = "Physics - Optics, Physics - Chemical Physics",

author = "Emiliano Cortes and Huidobro, {Paloma A.} and Sinclair, {Hugo G.} and Stina Guldbrand and Peveler, {William J.} and Timothy Davies and Simona Parrinello and Frederik G{\"o}rlitz and Chris Dunsby and Neil, {Mark A. A.} and Yonatan Sivan and Parkin, {Ivan P.} and French, {Paul M.} and Maier, {Stefan A.}",

year = "2016",

month = oct,

day = "5",

language = "אנגלית",

journal = "ACS Nano",

issn = "1936-0851",

publisher = "American Chemical Society",

}

TY - JOUR

T1 - Plasmonic nanoprobes for stimulated emission depletion microscopy

AU - Cortes, Emiliano

AU - Huidobro, Paloma A.

AU - Sinclair, Hugo G.

AU - Guldbrand, Stina

AU - Peveler, William J.

AU - Davies, Timothy

AU - Parrinello, Simona

AU - Görlitz, Frederik

AU - Dunsby, Chris

AU - Neil, Mark A. A.

AU - Sivan, Yonatan

AU - Parkin, Ivan P.

AU - French, Paul M.

AU - Maier, Stefan A.

PY - 2016/10/5

Y1 - 2016/10/5

N2 - Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles in order to enhance the stimulated depletion of excited molecules for super-resolved microscopy. We demonstrate stimulated emission depletion (STED) microscopy with gold nanorods with a long axis of only 26 nm and a width of 8 nm that provide an enhancement of the resolution compared to fluorescent-only probes without plasmonic components irradiated with the same depletion power. These novel nanoparticle-assisted STED probes represent a ~2x10^3 reduction in probe volume compared to previously used nanoparticles and we demonstrate their application to the first plasmon-assisted STED cellular imaging. We also discuss their current limitations.Comment: 30 pages, 15 figures, main text and supplementary informatio

AB - Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles in order to enhance the stimulated depletion of excited molecules for super-resolved microscopy. We demonstrate stimulated emission depletion (STED) microscopy with gold nanorods with a long axis of only 26 nm and a width of 8 nm that provide an enhancement of the resolution compared to fluorescent-only probes without plasmonic components irradiated with the same depletion power. These novel nanoparticle-assisted STED probes represent a ~2x10^3 reduction in probe volume compared to previously used nanoparticles and we demonstrate their application to the first plasmon-assisted STED cellular imaging. We also discuss their current limitations.Comment: 30 pages, 15 figures, main text and supplementary informatio

KW - Physics - Optics

KW - Physics - Chemical Physics

M3 - מאמר

SN - 1936-0851

JO - ACS Nano

JF - ACS Nano

ER -

Plasmonic nanoprobes for stimulated emission depletion microscopy

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Keywords

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