Plasmonic Nanoprobes for Stimulated Emission Depletion Nanoscopy

Emiliano Cortés; Paloma A. Huidobro; Hugo G. Sinclair; Stina Guldbrand; William J. Peveler; Timothy Davies; Simona Parrinello; Frederik Görlitz; Chris Dunsby; Mark A.A. Neil; Yonatan Sivan; Ivan P. Parkin; Paul M.W. French; Stefan A. Maier

doi:10.1021/acsnano.6b06361

Plasmonic Nanoprobes for Stimulated Emission Depletion Nanoscopy

Emiliano Cortés, Paloma A. Huidobro, Hugo G. Sinclair, Stina Guldbrand, William J. Peveler, Timothy Davies, Simona Parrinello, Frederik Görlitz, Chris Dunsby, Mark A.A. Neil, Yonatan Sivan, Ivan P. Parkin, Paul M.W. French, Stefan A. Maier

Unit of Photonics and Electro-Optics Engineering

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles in order to enhance the stimulated depletion of excited molecules for super-resolved nanoscopy. We demonstrate stimulated emission depletion (STED) nanoscopy with gold nanorods with a long axis of only 26 nm and a width of 8 nm. These particles provide an enhancement of up to 50% of the resolution compared to fluorescent-only probes without plasmonic components irradiated with the same depletion power. The nanoparticle-assisted STED probes reported here represent a ∼2 × 10³ reduction in probe volume compared to previously used nanoparticles. Finally, we demonstrate their application toward plasmon-assisted STED cellular imaging at low-depletion powers, and we also discuss their current limitations.

Original language	English
Pages (from-to)	10454-10461
Number of pages	8
Journal	ACS Nano
Volume	10
Issue number	11
DOIs	https://doi.org/10.1021/acsnano.6b06361
State	Published - 22 Nov 2016

Keywords

STED nanoscopy
bioimaging
nanorods
plasmonic nanoparticles
super resolution

ASJC Scopus subject areas

Materials Science (all)
Engineering (all)
Physics and Astronomy (all)

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10.1021/acsnano.6b06361

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@article{8f93c81e27b2492e980e2f705416081d,

title = "Plasmonic Nanoprobes for Stimulated Emission Depletion Nanoscopy",

abstract = "Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles in order to enhance the stimulated depletion of excited molecules for super-resolved nanoscopy. We demonstrate stimulated emission depletion (STED) nanoscopy with gold nanorods with a long axis of only 26 nm and a width of 8 nm. These particles provide an enhancement of up to 50% of the resolution compared to fluorescent-only probes without plasmonic components irradiated with the same depletion power. The nanoparticle-assisted STED probes reported here represent a ∼2 × 103 reduction in probe volume compared to previously used nanoparticles. Finally, we demonstrate their application toward plasmon-assisted STED cellular imaging at low-depletion powers, and we also discuss their current limitations.",

keywords = "STED nanoscopy, bioimaging, nanorods, plasmonic nanoparticles, super resolution",

author = "Emiliano Cort{\'e}s and Huidobro, {Paloma A.} and Sinclair, {Hugo G.} and Stina Guldbrand and Peveler, {William J.} and Timothy Davies and Simona Parrinello and Frederik G{\"o}rlitz and Chris Dunsby and Neil, {Mark A.A.} and Yonatan Sivan and Parkin, {Ivan P.} and French, {Paul M.W.} and Maier, {Stefan A.}",

note = "Funding Information: This project was supported by the MRC Next Generation Optical Microscopy Initiative, (MR/K015834/1), EPSRC Reactive Plasmonics project EP/M013812/1, the Royal Society, and the Leverhulme Trust. E.C. and P.A.H. are supported by Marie-Sklodowska Curie fellowships. W.P. is supported by an EPSRC Doctoral Prize Fellowship (EP/M506448/1). H.S. acknowledges a CASE Ph.D. studentship from the U.K. Engineering and Physical Sciences Research Council (EPSRC) and the Diamond Trading Company. Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",

year = "2016",

month = nov,

day = "22",

doi = "10.1021/acsnano.6b06361",

language = "English",

volume = "10",

pages = "10454--10461",

journal = "ACS Nano",

issn = "1936-0851",

publisher = "American Chemical Society",

number = "11",

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T1 - Plasmonic Nanoprobes for Stimulated Emission Depletion Nanoscopy

AU - Cortés, Emiliano

AU - Huidobro, Paloma A.

AU - Sinclair, Hugo G.

AU - Guldbrand, Stina

AU - Peveler, William J.

AU - Davies, Timothy

AU - Parrinello, Simona

AU - Görlitz, Frederik

AU - Dunsby, Chris

AU - Neil, Mark A.A.

AU - Sivan, Yonatan

AU - Parkin, Ivan P.

AU - French, Paul M.W.

AU - Maier, Stefan A.

N1 - Funding Information: This project was supported by the MRC Next Generation Optical Microscopy Initiative, (MR/K015834/1), EPSRC Reactive Plasmonics project EP/M013812/1, the Royal Society, and the Leverhulme Trust. E.C. and P.A.H. are supported by Marie-Sklodowska Curie fellowships. W.P. is supported by an EPSRC Doctoral Prize Fellowship (EP/M506448/1). H.S. acknowledges a CASE Ph.D. studentship from the U.K. Engineering and Physical Sciences Research Council (EPSRC) and the Diamond Trading Company. Publisher Copyright: © 2016 American Chemical Society.

PY - 2016/11/22

Y1 - 2016/11/22

N2 - Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles in order to enhance the stimulated depletion of excited molecules for super-resolved nanoscopy. We demonstrate stimulated emission depletion (STED) nanoscopy with gold nanorods with a long axis of only 26 nm and a width of 8 nm. These particles provide an enhancement of up to 50% of the resolution compared to fluorescent-only probes without plasmonic components irradiated with the same depletion power. The nanoparticle-assisted STED probes reported here represent a ∼2 × 103 reduction in probe volume compared to previously used nanoparticles. Finally, we demonstrate their application toward plasmon-assisted STED cellular imaging at low-depletion powers, and we also discuss their current limitations.

AB - Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles in order to enhance the stimulated depletion of excited molecules for super-resolved nanoscopy. We demonstrate stimulated emission depletion (STED) nanoscopy with gold nanorods with a long axis of only 26 nm and a width of 8 nm. These particles provide an enhancement of up to 50% of the resolution compared to fluorescent-only probes without plasmonic components irradiated with the same depletion power. The nanoparticle-assisted STED probes reported here represent a ∼2 × 103 reduction in probe volume compared to previously used nanoparticles. Finally, we demonstrate their application toward plasmon-assisted STED cellular imaging at low-depletion powers, and we also discuss their current limitations.

KW - STED nanoscopy

KW - bioimaging

KW - nanorods

KW - plasmonic nanoparticles

KW - super resolution

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EP - 10461

JO - ACS Nano

JF - ACS Nano

IS - 11

ER -

Plasmonic Nanoprobes for Stimulated Emission Depletion Nanoscopy

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