Poly (D,L-lactic-co-glycolide) nanoparticles for the improved therapeutic efficacy of all-trans-retinoic acid: A study of acute myeloid leukemia (AML) cell differentiation in vitro

Aswathy Mary Simon, Sankar Jagadeeshan, Emimol Abraham, Ashalatha Akhilandeshwaran, Jisha J. Pillai, Nisha Asok Kumar, Asha Nair Sivakumari, Gopalakrishnapillai Sankaramangalam Vinod Kumar

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

All-trans-retinoic acid reverses malignant cell growth and induces cell differentiation and apoptosis. Poor aqueous solubility and uncertain bioavailability are the limiting factors for using all-trans-retinoic acid for tumor therapy. The objective of present study was to encapsulate the hydrophobic drug all-trans-retinoic acid in the polymer poly (lactide-coglycolide). The encapsulation was expected to improve the bioavailability and solubility of the drug. Oil in water single emulsion solvent evaporation technique used for the preparation efficiently encapsulated about 60% of the drug. The drug release profile showed a biphasic pattern with 70% of the drug being released in first 48 hrs and the residual drug showing a slow controlled release reaching up to 8 days. The particle size of 150-200 nm as determined with TEM was ideal for tumor targeting. All-trans-retinoic acid loaded nanoparticles were efficient to induce differentiation and blocked the proliferation of HL-60 cells invitro. These studies also revealed that the dosage of drug required for the therapeutic effects have been reduced efficiently. Our studies thereby demonstrate that Poly (lactide-co-glycolide) based nanoparticles may be efficient for parenteral administration of the drug.

Original languageEnglish
Pages (from-to)805-810
Number of pages6
JournalMedicinal Chemistry
Volume8
Issue number5
DOIs
StatePublished - 1 Sep 2012
Externally publishedYes

Keywords

  • All-trans-retinoic acid
  • Cancer
  • Controlled release
  • HL-60
  • Myeloid leukemia
  • Nanoparticles

ASJC Scopus subject areas

  • Drug Discovery

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