Polyamine biosynthetic enzymes in Chlorella: Characterization of ornithine and arginine decarboxylase

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Abstract

In a Chlorella culture grown asynchronously under autotrophic conditions, two biosynthetic enzymes of putrescine-ornithine decarboxylase (ODC) and arginine decarboxylase (ADC)-were detected. Both enzymes require pyridoxal phosphate and dithiothreitol for their activity but differ in their optimal pH, the ionic strength of their buffer, temperature of inactivation, and Km. In addition, L-canaline was found to inhibit the activity of ODC but not that of ADC. During the logarithmic phase of growth, ODC activity increased sharply, then decreased before the onset of the stationary phase. ADC activity changed only slightly during growth.

Original languageEnglish
Pages (from-to)1003-1010
Number of pages8
JournalPlant and Cell Physiology
Volume24
Issue number6
StatePublished - 1 Sep 1983

Keywords

  • Arginine decarboxylase
  • Cell division
  • Chlorell
  • Ornithine decarboxylase
  • Polyamines

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