@article{1b9069d9f8ea40898b869b1ed7d5f6bf,
title = "Polycomb Repressive Complex 1 Controls Maintenance of Fungiform Papillae by Repressing Sonic Hedgehog Expression",
abstract = "How tissue patterns are formed and maintained are fundamental questions. The murine tongue epithelium, a paradigm for tissue patterning, consists of an array of specialized fungiform papillae structures that harbor taste cells. The formation of fungiform papillae is preceded by pronounced spatial changes in gene expression, in which taste cell genes such as Shh, initially diffused in lingual epithelial progenitors, become restricted to taste cells when their specification progresses. However, the requirement of spatial restriction of taste cell gene expression for patterning and formation of fungiform papillae is unknown. Here, we show that a chromatin regulator, Polycomb repressive complex (PRC) 1, is required for proper maintenance of fungiform papillae by repressing Shh and preventing ectopic SHH signaling in non-taste cells. Ablation of SHH signaling in PRC1-null non-taste cells rescues the maintenance of taste cells. Altogether, our studies exemplify how epigenetic regulation establishes spatial gene expression patterns necessary for specialized niche structures. Formation and maintenance of patterns are critical for tissue development. Bar et al. show that PRC1, an epigenetic regulator, is critical for lingual papillae development. Specifically, PRC1 regulates maintenance of the developing fungiform papillae, harboring taste cells, by repressing Shh expression in the non-gustatory epithelium surrounding taste cells.",
keywords = "PRC1, Polycomb, Sonic Hedgehog, filiform papillae, fungiform papillae",
author = "Carmit Bar and Idan Cohen and Dejian Zhao and Venu Pothula and Anna Litskevitch and Haruhiko Koseki and Deyou Zheng and Elena Ezhkova",
note = "Funding Information: For help, advice, critical suggestions, and/or reagents, we thank Sergei Ezhkov and Drs. Pooja Flora, Ya-Chieh Hsu, Sarah Millar, Jose Silva, John Mandeli, Julie Segre, and Elaine Fuchs. We thank Drs. Weipeng Mu and Terry Magnuson for Eed flox/flox mice, Dr. Miguel Vidal for Ring1a−/− and Ring1b flox/flox mice, and Dr. Philippe Soriano for Cdkn2a−/− mice. We thank the Flow Cytometry Core, the Imaging Facility, the Electron Microscopy Facility, and the Tisch Cancer Center sequencing facility at the ISMMS. C.B. is a Merksamer Fund scholar. I.C. is supported by a Training Program in Stem Cell Biology fellowship from the New York State Department of Health ( NYSTEM-C32561GG ). Research reported in this publication was supported by NIH/NIDCD grant R01DC017400 (to E.E.), NIH/NHBLI grant R01HL133120 (to D. Zheng), and the Tisch Cancer Institute under a P30 Cancer Support Grant (to E.E.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. Funding Information: For help, advice, critical suggestions, and/or reagents, we thank Sergei Ezhkov and Drs. Pooja Flora, Ya-Chieh Hsu, Sarah Millar, Jose Silva, John Mandeli, Julie Segre, and Elaine Fuchs. We thank Drs. Weipeng Mu and Terry Magnuson for Eed flox/flox mice, Dr. Miguel Vidal for Ring1a?/? and Ring1b flox/flox mice, and Dr. Philippe Soriano for Cdkn2a?/? mice. We thank the Flow Cytometry Core, the Imaging Facility, the Electron Microscopy Facility, and the Tisch Cancer Center sequencing facility at the ISMMS. C.B. is a Merksamer Fund scholar. I.C. is supported by a Training Program in Stem Cell Biology fellowship from the New York State Department of Health (NYSTEM-C32561GG). Research reported in this publication was supported by NIH/NIDCD grant R01DC017400 (to E.E.), NIH/NHBLI grant R01HL133120 (to D. Zheng), and the Tisch Cancer Institute under a P30 Cancer Support Grant (to E.E.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. Conceptualization, C.B. and E.E.; Methodology, C.B. and E.E.; Investigation, C.B. I.C. V.P. and A.L.; Resources, H.K.; Formal Analysis & Data Curation, C.B. D. Zhao, D. Zheng, and E.E.; Visualization, C.B.; Writing ? Original Draft, Review, & Editing, C.B. and E.E. with input from all authors; Supervision, Project Administration, & Funding Acquisition, E.E. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2019 The Author(s)",
year = "2019",
month = jul,
day = "2",
doi = "10.1016/j.celrep.2019.06.011",
language = "English",
volume = "28",
pages = "257--266.e5",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "1",
}