Possible involvement of calmodulin in the regulation of ATPase activity in guard cells

Ali Nejidat

doi:10.1111/j.1399-3054.1995.tb00946.x

Possible involvement of calmodulin in the regulation of ATPase activity in guard cells

Ali Nejidat

Zuckerberg Institute for Water Research

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Calcium ions play an important role in the regulation of stomatal movement and the mechanism underlying this action is yet to be determined. It is suggested that guard cell plasma membrane ATPase is a target for calcium action and that this effect is mediated by calmodulin. In this study, the effects of calcium and two calmodulin antagonists on ATPase activity in a crude homogenate of Commelina communis L. guard cell protoplasts were examined. The homogenate contained Mg²⁺‐dependent, K⁺‐simulated ATPase activity, which was inhibited by CaCl² while stimulated by the calmodulin antagonists, compound 48/80 and chlorpromazine. The calmodulin antagonists partially reversed the inhibitory effect of calcium ions. The results support the possibility of calmodulin involvement in the regulation of guard cell ATPase activity by calcium ions.

Original language	English
Pages (from-to)	411-414
Number of pages	4
Journal	Physiologia Plantarum
Volume	94
Issue number	3
DOIs	https://doi.org/10.1111/j.1399-3054.1995.tb00946.x
State	Published - 1 Jan 1995

Keywords

ATPase
Commelina communis
calcium
calmodulin
chlorpromazine
compound 48/80
stomata

ASJC Scopus subject areas

Physiology
Genetics
Plant Science
Cell Biology

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10.1111/j.1399-3054.1995.tb00946.x

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title = "Possible involvement of calmodulin in the regulation of ATPase activity in guard cells",

abstract = "Calcium ions play an important role in the regulation of stomatal movement and the mechanism underlying this action is yet to be determined. It is suggested that guard cell plasma membrane ATPase is a target for calcium action and that this effect is mediated by calmodulin. In this study, the effects of calcium and two calmodulin antagonists on ATPase activity in a crude homogenate of Commelina communis L. guard cell protoplasts were examined. The homogenate contained Mg2+‐dependent, K+‐simulated ATPase activity, which was inhibited by CaCl2 while stimulated by the calmodulin antagonists, compound 48/80 and chlorpromazine. The calmodulin antagonists partially reversed the inhibitory effect of calcium ions. The results support the possibility of calmodulin involvement in the regulation of guard cell ATPase activity by calcium ions.",

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AU - Nejidat, Ali

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AB - Calcium ions play an important role in the regulation of stomatal movement and the mechanism underlying this action is yet to be determined. It is suggested that guard cell plasma membrane ATPase is a target for calcium action and that this effect is mediated by calmodulin. In this study, the effects of calcium and two calmodulin antagonists on ATPase activity in a crude homogenate of Commelina communis L. guard cell protoplasts were examined. The homogenate contained Mg2+‐dependent, K+‐simulated ATPase activity, which was inhibited by CaCl2 while stimulated by the calmodulin antagonists, compound 48/80 and chlorpromazine. The calmodulin antagonists partially reversed the inhibitory effect of calcium ions. The results support the possibility of calmodulin involvement in the regulation of guard cell ATPase activity by calcium ions.

KW - ATPase

KW - Commelina communis

KW - calcium

KW - calmodulin

KW - chlorpromazine

KW - compound 48/80

KW - stomata

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Possible involvement of calmodulin in the regulation of ATPase activity in guard cells

Abstract

Keywords

ASJC Scopus subject areas

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