Possible therapeutic effect of magnesium sulfate in pre-eclampsia by the down-regulation of placental tumor necrosis factor-alpha secretion

Alaa Amash, Adi Y. Weintraub, Eyal Sheiner, Atef Zeadna, Mahmoud Huleihel, Gershon Holcberg

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Objective. To examine the effect of magnesium sulfate (MgSO4) on tumor necrosis factor-alpha (TNF-α) secretion by preeclamptic placentas. Study design. Cotyledons of six, term, normotensive and ten, pre-eclamptic placentas were dually perfused for six hours (6h), with MgSO4 (6-7 mg %) in the maternal reservoir [normotensive (n = 3); pre-eclamptic (n = 5)], and with control medium (without MgSO4) [normotensive (n = 3); pre-eclamptic (n = 5)]. Perfusate samples from the maternal and the fetal circulations were collected every 30 min throughout the 6h of perfusion, and examined for TNF-α levels using ELISA. Statistical significance was determined using a 2-way analysis of variance. Results. Pre-eclamptic placentas perfused with control medium (without MgSO4) secreted higher levels of TNF-α into the fetal and the maternal circulations (1.60 ± 0.59 pg/mL/g of cotyledon and 14.28 ± 2.69 pg/mL/g of cotyledon, respectively), as compared to the fetal and maternal circulations of normotensive placentas (0.25 ± 0.09 pg/mL/g of cotyledon and 6.73 ± 1.11 pg/mL/g of cotyledon, respectively) (p < 0.01). Addition of MgSO4 to normotensive placentas did not affect TNF-α levels in the fetal or maternal circulations. However, exposure of pre-eclamptic placentas to MgSO4 significantly decreased TNF-α levels in both the fetal (0.89 ± 0.09 pg/mL/g of cotyledon versus 1.6 ± 0.59 pg/mL/g of cotyledon; p < 0.05) and the maternal circulations (4.74 ± 2.78 pg/mL/g of cotyledon versus 14.28 ± 2.69 pg/mL/g of cotyledon; p < 0.01). Conclusion. Down-regulation of placental TNF-α secretion by MgSO4 in pre-eclampsia might indicate a possible therapeutic effect for this agent in reducing maternal, endothelial dysfunction and in improving neonatal outcome in pre-eclampsia, by reducing TNF-α levels in maternal and fetal circulations.

Original languageEnglish
Pages (from-to)58-64
Number of pages7
JournalEuropean Cytokine Network
Volume21
Issue number1
DOIs
StatePublished - 1 Mar 2010

Keywords

  • Magnesium sulfate
  • Placental perfusion
  • Pre-eclampsia
  • Tumor necrosis factor-alpha

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry

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