Practical Aspects of Fluorescence Analysis of Free Zinc Ion in Biological Systems: pZn for the Biologist

Michal Hershfinkel; Richard B. Thompson; J. Christopher; Carol A Fierke; Nissa M Westerberg; Rebecca A Bozym

doi:10.1201/9781420028287-19

Practical Aspects of Fluorescence Analysis of Free Zinc Ion in Biological Systems: pZn for the Biologist

Michal Hershfinkel, Richard B. Thompson, J. Christopher, Carol A Fierke, Nissa M Westerberg, Rebecca A Bozym

Department of Physiology and Cell Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

The biology of zinc has enjoyed a dramatic growth in attention over the last 20 years or so, as it has become apparent that zinc plays many roles in living systems in addition to serving as an enzymatic cofactor. At the same time, tools for studying zinc biology (mainly based on fluorescence) have been developed by us and others which have greatly aided our understanding of zinc in living systems of all kinds. However, zinc is maintained at very low free concentrations in biological systems, which makes the study of zinc biology challenging. In this chapter we confront some of these challenges and provide best practices for preparing zinc solutions and measuring zinc concentrations.

Original language	English
Title of host publication	Fluorescence Sensors and Biosensors
Editors	Richard B. Thompson
Publisher	CRC Press
Chapter	14
Pages	351-376
ISBN (Electronic)	9780429134449
ISBN (Print)	9780824727376
DOIs	https://doi.org/10.1201/9781420028287-19
State	Published - Dec 2005

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abstract = "The biology of zinc has enjoyed a dramatic growth in attention over the last 20 years or so, as it has become apparent that zinc plays many roles in living systems in addition to serving as an enzymatic cofactor. At the same time, tools for studying zinc biology (mainly based on fluorescence) have been developed by us and others which have greatly aided our understanding of zinc in living systems of all kinds. However, zinc is maintained at very low free concentrations in biological systems, which makes the study of zinc biology challenging. In this chapter we confront some of these challenges and provide best practices for preparing zinc solutions and measuring zinc concentrations.",

author = "Michal Hershfinkel and Thompson, {Richard B.} and J. Christopher and Fierke, {Carol A} and Westerberg, {Nissa M} and Bozym, {Rebecca A}",

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T2 - pZn for the Biologist

AU - Hershfinkel, Michal

AU - Thompson, Richard B.

AU - Christopher, J.

AU - Fierke, Carol A

AU - Westerberg, Nissa M

AU - Bozym, Rebecca A

PY - 2005/12

Y1 - 2005/12

N2 - The biology of zinc has enjoyed a dramatic growth in attention over the last 20 years or so, as it has become apparent that zinc plays many roles in living systems in addition to serving as an enzymatic cofactor. At the same time, tools for studying zinc biology (mainly based on fluorescence) have been developed by us and others which have greatly aided our understanding of zinc in living systems of all kinds. However, zinc is maintained at very low free concentrations in biological systems, which makes the study of zinc biology challenging. In this chapter we confront some of these challenges and provide best practices for preparing zinc solutions and measuring zinc concentrations.

AB - The biology of zinc has enjoyed a dramatic growth in attention over the last 20 years or so, as it has become apparent that zinc plays many roles in living systems in addition to serving as an enzymatic cofactor. At the same time, tools for studying zinc biology (mainly based on fluorescence) have been developed by us and others which have greatly aided our understanding of zinc in living systems of all kinds. However, zinc is maintained at very low free concentrations in biological systems, which makes the study of zinc biology challenging. In this chapter we confront some of these challenges and provide best practices for preparing zinc solutions and measuring zinc concentrations.

U2 - 10.1201/9781420028287-19

DO - 10.1201/9781420028287-19

M3 - Chapter

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SP - 351

EP - 376

BT - Fluorescence Sensors and Biosensors

A2 - Thompson, Richard B.

PB - CRC Press

ER -

Practical Aspects of Fluorescence Analysis of Free Zinc Ion in Biological Systems: pZn for the Biologist

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