Preimplantation HLA typing with aneuploidy testing

Svetlana Rechitsky, Anver Kuliev, Tatyana Sharapova, Katya Lazuik, Seckin Ozen, Irina Barksy, Oleg Verlinsky, Ilan Tur-Kaspa, Yury Verlinsky

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Preimplantation HLA typing has been introduced for the treatment of affected siblings, requiring HLA-identical stem cell transplantation. This was applied either in combination with preimplantation genetic diagnosis (PGD) to ensure that the preselected HLA-matched embryos were also free of the genetic disorder, or without PGD, with the only purpose of selecting and transferring the HLA-matched embryos. Because patients requesting preimplantation HLA typing are usually of advanced reproductive age, aneuploidy testing allows not only the avoidance of the birth of children with chromosomal disorders, but also improvement of the reproductive outcome, which is still not sufficiently high in preimplantation HLA typing at the present time. This study presents the results of the first experience of preimplantation HLA typing combined with aneuploidy testing, demonstrating feasibility and impact of aneuploidy testing on the accuracy and outcome of preimplantation HLA typing. Of a total of 138 cycles performed, 87 were combined with PGD and 52 without testing for the causative gene, of which aneuploidy testing was performed in 27 cycles, allowing the preselection and transfer of only those HLA-matched embryos that were also euploid. Although the euploid HLA-identical embryos were available for transfer in only half of these cycles, pregnancy and birth of unaffected HLA-identical children were observed in approximately half of these cycles, suggesting the potential usefulness of incorporating aneuploidy testing into preimplantation HLA typing.

Original languageEnglish
Article number1993
Pages (from-to)89-100
Number of pages12
JournalReproductive BioMedicine Online
Volume12
Issue number1
DOIs
StatePublished - 1 Jan 2006
Externally publishedYes

Keywords

  • Accucary
  • PCR-based aneuploidy testing
  • PGD
  • Preimplantation HLA typing
  • Reproductive outcome

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Developmental Biology

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