Preparation of tritium-labeled hyaluronic acid oligomers and their use in enzyme studies

Stefan Highsmith, David M. Chipman

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Tritium-labeled oligosaccharides can be prepared from hyaluronic acid by the Wilzbach technique with greater ease than usual by use of the specificity of hyaluronidase in the course of purification. Labeled oligomers of hyalobiuronic acid were isolated and shown to be radiochemically homogeneous, and are used in studies of complex enzymic kinetics. The techniques used may have general use in preparing generally labeled oligosaccharides.

Original languageEnglish
Pages (from-to)557-566
Number of pages10
JournalAnalytical Biochemistry
Volume61
Issue number2
DOIs
StatePublished - 1 Jan 1974

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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