TY - JOUR
T1 - Prevascularization of cardiac patch on the omentum improves its therapeutic outcome
AU - Dvir, Tal
AU - Kedem, Alon
AU - Ruvinov, Emil
AU - Levy, Oren
AU - Freeman, Inbar
AU - Landa, Natalie
AU - Holbova, Radka
AU - Feinberg, Micha S.
AU - Dror, Shani
AU - Etzion, Yoram
AU - Leor, Jonathan
AU - Cohen, Smadar
PY - 2009/9/1
Y1 - 2009/9/1
N2 - The recent progress made in the bioengineering of cardiac patches offers a new therapeutic modality for regenerating the myocardium after myocardial infarction (MI). We present here a strategy for the engineering of a cardiac patch with mature vasculature by heterotopic transplantation onto the omentum. The patch was constructed by seeding neonatal cardiac cells with a mixture of prosurvival and angiogenic factors into an alginate scaffold capable of factor binding and sustained release. After 48 h in culture, the patch was vascularized for 7 days on the omentum, then explanted and transplanted onto infarcted rat hearts, 7 days after MI induction. When evaluated 28 days later, the vascularized cardiac patch showed structural and electrical integration into host myocardium. Moreover, the vascularized patch induced thicker scars, prevented further dilatation of the chamber and ventricular dysfunction. Thus, our study provides evidence that grafting prevascularized cardiac patch into infarct can improve cardiac function after MI.
AB - The recent progress made in the bioengineering of cardiac patches offers a new therapeutic modality for regenerating the myocardium after myocardial infarction (MI). We present here a strategy for the engineering of a cardiac patch with mature vasculature by heterotopic transplantation onto the omentum. The patch was constructed by seeding neonatal cardiac cells with a mixture of prosurvival and angiogenic factors into an alginate scaffold capable of factor binding and sustained release. After 48 h in culture, the patch was vascularized for 7 days on the omentum, then explanted and transplanted onto infarcted rat hearts, 7 days after MI induction. When evaluated 28 days later, the vascularized cardiac patch showed structural and electrical integration into host myocardium. Moreover, the vascularized patch induced thicker scars, prevented further dilatation of the chamber and ventricular dysfunction. Thus, our study provides evidence that grafting prevascularized cardiac patch into infarct can improve cardiac function after MI.
KW - Affinity-binding alginate scaffolds
KW - Cardiac tissue engineering
KW - Myocardial infarction
KW - SDF-1
KW - Vascularization
UR - http://www.scopus.com/inward/record.url?scp=70349272259&partnerID=8YFLogxK
U2 - 10.1073/pnas.0812242106
DO - 10.1073/pnas.0812242106
M3 - Article
C2 - 19706385
AN - SCOPUS:70349272259
SN - 0027-8424
VL - 106
SP - 14990
EP - 14995
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 35
ER -