Prevention of Prostaglandin E2-induced desensitization of rat ovarian cyclic AMP response by concanavalin A

B. Strulovici, Yacob Weinstein, U. Zor

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Prolonged exposure (> 6 h) of cultured granulosa cells to Prostaglandin E2 (PGE2; 1 μg/ml) led to a near-total loss of the cyclic AMP response to subsequent addition of fresh hormone. Pre-treatment of the cells with concanavalin A (ConA; 2.0 μg/ml) for 1 h blocked the desensitizing action of PGE2, so that the decline in the response was reduced by 60% with the hormone at high concentration (1.0 μg/ml); a full response was preserved at submaximal concentration of PGE2 (0.1 - 0.3μg/ml). Other lectins (succinyl Con a, peanut agglutinin and, to a lesser extent, phytohemagglutinin and wheat germ agglutinin) had a stabilizing effect similar to that of Con A. Addition of alpha-methyl-mannoside either with Con A or various times following the addition of Con A to the cells prevented the protective effect of Con A. Concomitant treatment with colchicine or cytochalasin B abolished the ability of Con A to prevent PGE2-induced desensitization.

Original languageEnglish
Pages (from-to)638-646
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume103
Issue number2
DOIs
StatePublished - 30 Nov 1981
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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