TY - JOUR
T1 - Probiotic Characteristics of Lactiplantibacillus Plantarum N-1 and Its Cholesterol-Lowering Effect in Hypercholesterolemic Rats
AU - Tian, Lei
AU - Liu, Rongmei
AU - Zhou, Zhiwei
AU - Xu, Xiaofang
AU - Feng, Su
AU - Kushmaro, Ariel
AU - Marks, Robert S.
AU - Wang, Dan
AU - Sun, Qun
N1 - Funding Information:
This work was supported by the National Key Research and Development Projects (2019YFE0103800), Sichuan Science and Technology Program (2021ZHFP0045, 2021YFN0092, 2022YFN0021), International Research and Development Program of Sichuan (2019YFH0113, 2021YFH0060, 2021YFH0072), the Fundamental Research Funds for the Central Universities (2020CDLZ-17), and the National University Student Innovation Experimental Project [201913705030].
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022/4/1
Y1 - 2022/4/1
N2 - In this study, the probiotic potential and treatment effects of Lactiplantibacillus plantarum N-1 in hypercholesterolemic rats were investigated, and the possible regulatory mechanisms of lipid metabolism via short-chain fatty acids (SCFAs) and 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase were elucidated. The strain N-1 displayed probiotic properties of antioxidant capacity, adhesion to Caco-2 cells, susceptibility to antibiotics in vitro. The results in animal study showed that the total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels in serum and TC in liver declined significantly in both N-1 and simvastatin (Sta) treatment groups compared to the control (P < 0.05), and the extent of these decreases were similar between them. The expression of the HMG-CoA gene in the N-1 group was downregulated significantly by 31.18% compared to the control (P < 0.01), and the contents of butyrate and valerate in N-1 groups were significantly higher than those in both model and Sta group (P < 0.05). Thus, promoting the production of the intestinal SCFAs and inhibiting the expression of HMG-CoA reductase by L. plantarum N-1 may contribute to the improved lipid metabolism and thus lowering cholesterol level in rats. Our investigation indicated that L. plantarum N-1 has the potential to be developed into a functional food supplement for hypercholesterolemia treatment.
AB - In this study, the probiotic potential and treatment effects of Lactiplantibacillus plantarum N-1 in hypercholesterolemic rats were investigated, and the possible regulatory mechanisms of lipid metabolism via short-chain fatty acids (SCFAs) and 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase were elucidated. The strain N-1 displayed probiotic properties of antioxidant capacity, adhesion to Caco-2 cells, susceptibility to antibiotics in vitro. The results in animal study showed that the total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels in serum and TC in liver declined significantly in both N-1 and simvastatin (Sta) treatment groups compared to the control (P < 0.05), and the extent of these decreases were similar between them. The expression of the HMG-CoA gene in the N-1 group was downregulated significantly by 31.18% compared to the control (P < 0.01), and the contents of butyrate and valerate in N-1 groups were significantly higher than those in both model and Sta group (P < 0.05). Thus, promoting the production of the intestinal SCFAs and inhibiting the expression of HMG-CoA reductase by L. plantarum N-1 may contribute to the improved lipid metabolism and thus lowering cholesterol level in rats. Our investigation indicated that L. plantarum N-1 has the potential to be developed into a functional food supplement for hypercholesterolemia treatment.
KW - 3-Hydroxy-3-methylglutaryl coenzyme A
KW - Cholesterol-lowering
KW - Lactiplantibacillus plantarum
KW - Probiotic
KW - Short-chain fatty acids
UR - http://www.scopus.com/inward/record.url?scp=85123467136&partnerID=8YFLogxK
U2 - 10.1007/s12602-021-09886-1
DO - 10.1007/s12602-021-09886-1
M3 - Article
C2 - 35064922
AN - SCOPUS:85123467136
SN - 1867-1306
VL - 14
SP - 337
EP - 348
JO - Probiotics and Antimicrobial Proteins
JF - Probiotics and Antimicrobial Proteins
IS - 2
ER -