Production of channel catfish (Ictalurus punctatus) recombinant gonadotropins using the S2 Drosophila cell line system

N. Zmora; S. Kumar; Y. Kazeto; J. M. Trant

doi:10.1023/B:FISH.0000030633.93255.46

Production of channel catfish (Ictalurus punctatus) recombinant gonadotropins using the S2 Drosophila cell line system

N. Zmora
, S. Kumar
, Y. Kazeto
, J. M. Trant

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The S2 cell system was utilized for the production of recombinant luteinizing hormone (LH) and follicle stimulating hormone (FSH) of the channel catfish (Ictalurus punctatus) as C-terminal His-tagged proteins. When expressed individually, the common α-subunit was secreted in abundance but both β-LH and β-FSH were poorly expressed. However, co-expression of the α-subunit with each of the β subunits using a duel promoter vector resulted in the abundant secretion of LH and FSH α/β heterodimers. These recombinant gonadotropins (GtH) were able to stimulate estradiol secretion in an ovarian follicle bioassay and activate recombinant gonadotropin receptors.

Original language	English
Pages (from-to)	475-477
Number of pages	3
Journal	Fish Physiology and Biochemistry
Volume	28
Issue number	1-4
DOIs	https://doi.org/10.1023/B:FISH.0000030633.93255.46
State	Published - 1 Dec 2003
Externally published	Yes

Keywords

FSH
Heterologous expression system
LH
Recombinant protein

ASJC Scopus subject areas

Biochemistry
Physiology
Aquatic Science

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10.1023/B:FISH.0000030633.93255.46

Cite this

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title = "Production of channel catfish (Ictalurus punctatus) recombinant gonadotropins using the S2 Drosophila cell line system",

abstract = "The S2 cell system was utilized for the production of recombinant luteinizing hormone (LH) and follicle stimulating hormone (FSH) of the channel catfish (Ictalurus punctatus) as C-terminal His-tagged proteins. When expressed individually, the common α-subunit was secreted in abundance but both β-LH and β-FSH were poorly expressed. However, co-expression of the α-subunit with each of the β subunits using a duel promoter vector resulted in the abundant secretion of LH and FSH α/β heterodimers. These recombinant gonadotropins (GtH) were able to stimulate estradiol secretion in an ovarian follicle bioassay and activate recombinant gonadotropin receptors.",

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year = "2003",

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TY - JOUR

T1 - Production of channel catfish (Ictalurus punctatus) recombinant gonadotropins using the S2 Drosophila cell line system

AU - Zmora, N.

AU - Kumar, S.

AU - Kazeto, Y.

AU - Trant, J. M.

PY - 2003/12/1

Y1 - 2003/12/1

N2 - The S2 cell system was utilized for the production of recombinant luteinizing hormone (LH) and follicle stimulating hormone (FSH) of the channel catfish (Ictalurus punctatus) as C-terminal His-tagged proteins. When expressed individually, the common α-subunit was secreted in abundance but both β-LH and β-FSH were poorly expressed. However, co-expression of the α-subunit with each of the β subunits using a duel promoter vector resulted in the abundant secretion of LH and FSH α/β heterodimers. These recombinant gonadotropins (GtH) were able to stimulate estradiol secretion in an ovarian follicle bioassay and activate recombinant gonadotropin receptors.

AB - The S2 cell system was utilized for the production of recombinant luteinizing hormone (LH) and follicle stimulating hormone (FSH) of the channel catfish (Ictalurus punctatus) as C-terminal His-tagged proteins. When expressed individually, the common α-subunit was secreted in abundance but both β-LH and β-FSH were poorly expressed. However, co-expression of the α-subunit with each of the β subunits using a duel promoter vector resulted in the abundant secretion of LH and FSH α/β heterodimers. These recombinant gonadotropins (GtH) were able to stimulate estradiol secretion in an ovarian follicle bioassay and activate recombinant gonadotropin receptors.

KW - FSH

KW - Heterologous expression system

KW - LH

KW - Recombinant protein

UR - https://www.scopus.com/pages/publications/8844221918

U2 - 10.1023/B:FISH.0000030633.93255.46

DO - 10.1023/B:FISH.0000030633.93255.46

M3 - Article

AN - SCOPUS:8844221918

SN - 0920-1742

VL - 28

SP - 475

EP - 477

JO - Fish Physiology and Biochemistry

JF - Fish Physiology and Biochemistry

IS - 1-4

ER -

Production of channel catfish (Ictalurus punctatus) recombinant gonadotropins using the S2 Drosophila cell line system

Abstract

Keywords

ASJC Scopus subject areas

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