Production of eicosapentaenoic acid (EPA) in Monodus subterraneus grown in a helical tubular photobioreactor as affected by cell density and light intensity

Congming Lu, Krishna Rao, David Hall, Avigad Vonshak

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

The effect of cell density (1-4.5 g L-1) and light intensity (44 and 82 μmol m-2 s-1) on fatty acid composition and eicosapentaenoic acid (EPA, 20:5 ω3) production was studied in a semi-continuous culture of Monodus subterraneus grown in a helical tubular photobioreactor ('Biocoil') under laboratory conditions. Under low light, the highest proportion of EPA (31.5% of total fatty acids) and EPA content (3.5% of dry weight), biomass productivity (1.3 g L-1 24 h-1) and EPA productivity (44 mg L-1 24 h-1) occurred at optimal cell density of about 1.7 g L-1. Cell density had no effect on the total fatty acid (TFA) content and was maintained at ca. 11% of dry weight. Under high light, the highest proportion of EPA to fatty acids (31.8%), the total fatty acids content (13.4%) and EPA content (4.3% of dry weight) occurred at cell density of about 3.4g L-1. But the highest biomass productivity (1.7 g L-1 24 h-1) and EPA productivity (56 mg L-1 24 h-1) were obtained at a cell density of 1.6 and 2.6g L-1, respectively. Our results suggest that manipulating the cell density and light intensity can modify the composition of fatty acid and production of eicosapentaenoic acid (EPA) in M. subterraneus.

Original languageEnglish
Pages (from-to)517-522
Number of pages6
JournalJournal of Applied Phycology
Volume13
Issue number6
DOIs
StatePublished - 31 Dec 2001

Keywords

  • Cell density
  • Eicosapentaenoic acid (EPA)
  • Light intensity
  • Monodus subterraneus
  • Polyunsaturated fatty acid

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