Production of the bioinsecticide Bacillus thuringiensis subsp. israelensis with deltamethrin increases toxicity towards mosquito larvae

G. Tetreau, C. D. Patil, A. Chandor-Proust, B. K. Salunke, S. V. Patil, L. Després

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Bacillus thuringiensis subsp. israelensis is a bioinsecticide used for larval mosquito control and it represents a safe alternative to chemical insecticides. Despite its environmental safety, it is less efficient and persistent than chemical insecticides. To bypass these limitations, we propose to combine the advantages of chemical and biological insecticides by producing Bti in a medium supplemented with a chemical insecticide (DDT, deltamethrin, permethrin, propoxur or temephos). Among the investigated insecticides, the addition of deltamethrin in the medium induced a higher toxicity (over 6·72-fold) of the composite deltamethrin-Bti towards mosquito larvae as compared to Bti alone. This was mainly due to the insertion of deltamethrin into the membranes of Bti spores, as evidenced by a quantification of membrane-extracted deltamethrin by HPLC. This composite larvicide is a promising tool to decrease the quantity of chemicals dispersed in the environment, to increase the efficacy of Bti and to facilitate its widespread use as a transition between chemical and biological insecticides. Further experiments are required to characterize the mechanisms that underline the incorporation of deltamethrin into Bti to optimize the production and the toxicity of this composite larvicide.

Original languageEnglish
Pages (from-to)151-156
Number of pages6
JournalLetters in Applied Microbiology
Volume57
Issue number2
DOIs
StatePublished - 1 Jan 2013
Externally publishedYes

Keywords

  • Aedes aegypti
  • Bioassays
  • Chemical insecticide
  • HPLC
  • Improved efficacy
  • Pyrethroid

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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