Profile and persistence of the virus-specific neutralizing humoral immune response in human survivors of sudan ebolavirus (Gulu)

Ariel Sobarzo, Allison Groseth, Olga Dolnik, Stephan Becker, Julius Julian Lutwama, Eddie Perelman, Victoria Yavelsky, Majidat Muhammad, Ana I. Kuehne, Robert S. Marks, John M. Dye, Leslie Lobel

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

To better understand humoral immunity following ebolavirus infection, a serological study of the humoral immune response against the individual viral proteins of Sudan ebolavirus (Gulu) in human survivors was performed. An enzyme-linked immunosorbent assay specific for full-length recombinant viral proteins NP, VP30, VP40, and GP1-649 (GP lacking the transmembrane domain) of Sudan ebolavirus (Gulu) was used as well as a plaque reduction neutralization test. Serum samples from human survivors, which were collected up to 10 years following recovery, were screened and analyzed. Results demonstrate that samples obtained 10 years following infection contain virus-specific antibodies that can neutralize virus. Neutralization correlates well with immunoreactivity against the viral proteins NP, VP30, and GP1-649. Sera from individuals who died or those with no documented infection but immunoreactive to ebolavirus did not neutralize. This work provides insight into the duration, profile of immunoreactivity, and neutralization capacity of the humoral immune response in ebolavirus survivors.

Original languageEnglish
Pages (from-to)299-309
Number of pages11
JournalJournal of Infectious Diseases
Volume208
Issue number2
DOIs
StatePublished - 15 Jul 2013

Keywords

  • antibodies
  • ebolavirus
  • epitopes
  • immune response
  • sera screening
  • serological profiling

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