Profiling of a few immune responsive genes expressed in postlarvae of Fenneropenaeus indicus challenged with Vibrio harveyi D3

S. Nayak; K. M. Ajay; N. Ramaiah; Ram M. Meena; R. A. Sreepada

doi:10.1016/j.jip.2011.04.001

Profiling of a few immune responsive genes expressed in postlarvae of Fenneropenaeus indicus challenged with Vibrio harveyi D3

S. Nayak, K. M. Ajay, N. Ramaiah, Ram M. Meena, R. A. Sreepada

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

We identified 38 genes and eight hypothetical proteins by sequencing of 1200 clones from a Vibrio harveyi challenged Fenneropenaeus indicus subtracted cDNA library. Based on physiological roles and functions these genes were categorized into 10 groups with ∼29% of the sequences having no matches in the databases. Immune related transcripts in the library were carboxypeptidase B, ferritin, translationally controlled tumor proteins (TCTP), hemocyanin, chitinase and serine carboxy peptidase. Remarkably, qPCR results imply 4.15, 3.45 and 1.86-fold rises in expression of ferritin, TCTP and hemocyanin transcripts respectively. Additionally, minor upregulation of other immune relevant genes lectin, penaeidin, crustin, MnSOD was observed in the challenged postlarvae.

Original language	English
Pages (from-to)	168-172
Number of pages	5
Journal	Journal of Invertebrate Pathology
Volume	107
Issue number	2
DOIs	https://doi.org/10.1016/j.jip.2011.04.001
State	Published - 1 Jun 2011
Externally published	Yes

Keywords

EST
Fenneropenaeus indicus
Ferritin
Penaeidin
SSH
Vibrio harveyi

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics

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10.1016/j.jip.2011.04.001

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title = "Profiling of a few immune responsive genes expressed in postlarvae of Fenneropenaeus indicus challenged with Vibrio harveyi D3",

abstract = "We identified 38 genes and eight hypothetical proteins by sequencing of 1200 clones from a Vibrio harveyi challenged Fenneropenaeus indicus subtracted cDNA library. Based on physiological roles and functions these genes were categorized into 10 groups with ∼29% of the sequences having no matches in the databases. Immune related transcripts in the library were carboxypeptidase B, ferritin, translationally controlled tumor proteins (TCTP), hemocyanin, chitinase and serine carboxy peptidase. Remarkably, qPCR results imply 4.15, 3.45 and 1.86-fold rises in expression of ferritin, TCTP and hemocyanin transcripts respectively. Additionally, minor upregulation of other immune relevant genes lectin, penaeidin, crustin, MnSOD was observed in the challenged postlarvae.",

keywords = "EST, Fenneropenaeus indicus, Ferritin, Penaeidin, SSH, Vibrio harveyi",

author = "S. Nayak and Ajay, {K. M.} and N. Ramaiah and Meena, {Ram M.} and Sreepada, {R. A.}",

year = "2011",

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doi = "10.1016/j.jip.2011.04.001",

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journal = "Journal of Invertebrate Pathology",

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AU - Nayak, S.

AU - Ajay, K. M.

AU - Ramaiah, N.

AU - Meena, Ram M.

AU - Sreepada, R. A.

PY - 2011/6/1

Y1 - 2011/6/1

N2 - We identified 38 genes and eight hypothetical proteins by sequencing of 1200 clones from a Vibrio harveyi challenged Fenneropenaeus indicus subtracted cDNA library. Based on physiological roles and functions these genes were categorized into 10 groups with ∼29% of the sequences having no matches in the databases. Immune related transcripts in the library were carboxypeptidase B, ferritin, translationally controlled tumor proteins (TCTP), hemocyanin, chitinase and serine carboxy peptidase. Remarkably, qPCR results imply 4.15, 3.45 and 1.86-fold rises in expression of ferritin, TCTP and hemocyanin transcripts respectively. Additionally, minor upregulation of other immune relevant genes lectin, penaeidin, crustin, MnSOD was observed in the challenged postlarvae.

AB - We identified 38 genes and eight hypothetical proteins by sequencing of 1200 clones from a Vibrio harveyi challenged Fenneropenaeus indicus subtracted cDNA library. Based on physiological roles and functions these genes were categorized into 10 groups with ∼29% of the sequences having no matches in the databases. Immune related transcripts in the library were carboxypeptidase B, ferritin, translationally controlled tumor proteins (TCTP), hemocyanin, chitinase and serine carboxy peptidase. Remarkably, qPCR results imply 4.15, 3.45 and 1.86-fold rises in expression of ferritin, TCTP and hemocyanin transcripts respectively. Additionally, minor upregulation of other immune relevant genes lectin, penaeidin, crustin, MnSOD was observed in the challenged postlarvae.

KW - EST

KW - Fenneropenaeus indicus

KW - Ferritin

KW - Penaeidin

KW - SSH

KW - Vibrio harveyi

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AN - SCOPUS:79955920830

SN - 0022-2011

VL - 107

SP - 168

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JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

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ER -

Profiling of a few immune responsive genes expressed in postlarvae of Fenneropenaeus indicus challenged with Vibrio harveyi D3

Abstract

Keywords

ASJC Scopus subject areas

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