Promoter activity of the 5' flanking region of a tobacco glycolate oxidase gene in transgenic tobacco plants

Simon Barak, Ali Nejidat, Micha Volokita

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A clone harboring the 5' flanking region of a tobacco glycolate oxidase (GLO) gene was isolated from a λEMBL3-tobacco genomic DNA libarary. Primer extension analysis indicated two major transcripts with 76 and 81 bp 5' UTRs. An RT-PCR assay mapped the major mRNA transcription initiation site to thymine at position 81 upstream of the translation initiation codon. A putative TATA box spanning positions -56 to -50 upstream of the transcription initiation site was found. Promoter activity of the 5' flanking region (-3.0 kb to +82 bp) was demonstrated in tobacco plants transformed with a GLO-β- glucuronidase (GUS) chimeric gene. Furthermore, in these transgenic plants, GUS expression patterns mimicked the expression patterns of the endogenous GLO.

Original languageEnglish
Pages (from-to)105-110
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1399
Issue number1
DOIs
StatePublished - 30 Jul 1998

Keywords

  • Glycolate oxidase
  • Light- dependent expression
  • Promoter
  • Transcription initiation site
  • Transgenic plant

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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