Promoter activity of the 5' flanking region of a tobacco glycolate oxidase gene in transgenic tobacco plants

Simon Barak; Ali Nejidat; Micha Volokita

doi:10.1016/S0167-4781(98)00083-9

Promoter activity of the 5' flanking region of a tobacco glycolate oxidase gene in transgenic tobacco plants

Simon Barak, Ali Nejidat, Micha Volokita

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A clone harboring the 5' flanking region of a tobacco glycolate oxidase (GLO) gene was isolated from a λEMBL3-tobacco genomic DNA libarary. Primer extension analysis indicated two major transcripts with 76 and 81 bp 5' UTRs. An RT-PCR assay mapped the major mRNA transcription initiation site to thymine at position 81 upstream of the translation initiation codon. A putative TATA box spanning positions -56 to -50 upstream of the transcription initiation site was found. Promoter activity of the 5' flanking region (-3.0 kb to +82 bp) was demonstrated in tobacco plants transformed with a GLO-β- glucuronidase (GUS) chimeric gene. Furthermore, in these transgenic plants, GUS expression patterns mimicked the expression patterns of the endogenous GLO.

Original language	English
Pages (from-to)	105-110
Number of pages	6
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1399
Issue number	1
DOIs	https://doi.org/10.1016/S0167-4781(98)00083-9
State	Published - 30 Jul 1998

Keywords

Glycolate oxidase
Light- dependent expression
Promoter
Transcription initiation site
Transgenic plant

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(98)00083-9

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@article{5205ac8f9d9b4d8d88be0562609b954f,

title = "Promoter activity of the 5' flanking region of a tobacco glycolate oxidase gene in transgenic tobacco plants",

abstract = "A clone harboring the 5' flanking region of a tobacco glycolate oxidase (GLO) gene was isolated from a λEMBL3-tobacco genomic DNA libarary. Primer extension analysis indicated two major transcripts with 76 and 81 bp 5' UTRs. An RT-PCR assay mapped the major mRNA transcription initiation site to thymine at position 81 upstream of the translation initiation codon. A putative TATA box spanning positions -56 to -50 upstream of the transcription initiation site was found. Promoter activity of the 5' flanking region (-3.0 kb to +82 bp) was demonstrated in tobacco plants transformed with a GLO-β- glucuronidase (GUS) chimeric gene. Furthermore, in these transgenic plants, GUS expression patterns mimicked the expression patterns of the endogenous GLO.",

keywords = "Glycolate oxidase, Light- dependent expression, Promoter, Transcription initiation site, Transgenic plant",

author = "Simon Barak and Ali Nejidat and Micha Volokita",

note = "Funding Information: We would like to thank Gila Granot for excellent technical assistance. A.N. was supported by a Deichmann fellowship. This study was supported in part by the Israel Science Foundation founded by the Israel Academy of Sciences and Humanities.",

year = "1998",

month = jul,

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doi = "10.1016/S0167-4781(98)00083-9",

language = "English",

volume = "1399",

pages = "105--110",

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publisher = "Elsevier B.V.",

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T1 - Promoter activity of the 5' flanking region of a tobacco glycolate oxidase gene in transgenic tobacco plants

AU - Barak, Simon

AU - Nejidat, Ali

AU - Volokita, Micha

N1 - Funding Information: We would like to thank Gila Granot for excellent technical assistance. A.N. was supported by a Deichmann fellowship. This study was supported in part by the Israel Science Foundation founded by the Israel Academy of Sciences and Humanities.

PY - 1998/7/30

Y1 - 1998/7/30

N2 - A clone harboring the 5' flanking region of a tobacco glycolate oxidase (GLO) gene was isolated from a λEMBL3-tobacco genomic DNA libarary. Primer extension analysis indicated two major transcripts with 76 and 81 bp 5' UTRs. An RT-PCR assay mapped the major mRNA transcription initiation site to thymine at position 81 upstream of the translation initiation codon. A putative TATA box spanning positions -56 to -50 upstream of the transcription initiation site was found. Promoter activity of the 5' flanking region (-3.0 kb to +82 bp) was demonstrated in tobacco plants transformed with a GLO-β- glucuronidase (GUS) chimeric gene. Furthermore, in these transgenic plants, GUS expression patterns mimicked the expression patterns of the endogenous GLO.

AB - A clone harboring the 5' flanking region of a tobacco glycolate oxidase (GLO) gene was isolated from a λEMBL3-tobacco genomic DNA libarary. Primer extension analysis indicated two major transcripts with 76 and 81 bp 5' UTRs. An RT-PCR assay mapped the major mRNA transcription initiation site to thymine at position 81 upstream of the translation initiation codon. A putative TATA box spanning positions -56 to -50 upstream of the transcription initiation site was found. Promoter activity of the 5' flanking region (-3.0 kb to +82 bp) was demonstrated in tobacco plants transformed with a GLO-β- glucuronidase (GUS) chimeric gene. Furthermore, in these transgenic plants, GUS expression patterns mimicked the expression patterns of the endogenous GLO.

KW - Glycolate oxidase

KW - Light- dependent expression

KW - Promoter

KW - Transcription initiation site

KW - Transgenic plant

UR - http://www.scopus.com/inward/record.url?scp=0032581570&partnerID=8YFLogxK

U2 - 10.1016/S0167-4781(98)00083-9

DO - 10.1016/S0167-4781(98)00083-9

M3 - Article

AN - SCOPUS:0032581570

SN - 0167-4781

VL - 1399

SP - 105

EP - 110

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 1

ER -

Promoter activity of the 5' flanking region of a tobacco glycolate oxidase gene in transgenic tobacco plants

Abstract

Keywords

ASJC Scopus subject areas

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