TY - JOUR
T1 - Prooxidant and anti-inflammatory potential of Garcinia xanthochymus fruit and its phytochemical characterisation by UHPLC-Q-Orbitrap HRMS
AU - Hati Boruah, Jyoti Lakshmi
AU - Puro, K. Nusalu
AU - Das, Deep Jyoti
AU - Gogoi, Parishmita
AU - Gogoi, Moloya
AU - Biswas, Anupam
AU - Famhawite, Vanlalhruaii
AU - Barman, Dipankar
AU - Mridha, Prosenjit
AU - Gajbhiye, Rahul
AU - Baishya, Rinku
N1 - Publisher Copyright:
© 2024 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2024/1/1
Y1 - 2024/1/1
N2 - Pro-oxidants play a crucial role in cancer by causing oxidative stress that leads to apoptosis. The present study demonstrates the prooxidant and anti-inflammatory potential of ethyl acetate and methanolic extracts of Garcinia xanthochymus fruit. Oxidation of Trolox and NADH activity indicated the pro-oxidant capacity of the extracts. Significant decrease in cell viability in B16F10 and MDA-MB-231 cancer cell lines and significant increase in caspase 3 activity after treatment with extracts indicated pro-oxidant induced apoptosis. Pre-treatment with the extracts significantly inhibited ROS, reduced NO production, inhibited LPS-induced COX-2 and suppressed IL-6 and TNF-α expression. HRMS analysis showed the presence of compounds like biflavonoids, xanthones, phloroglucinols, benzophenones, etc. The fruit is rich in total phenolic and flavonoid contents, and have DPPH radical scavenging, ferric reducing antioxidant and metal chelating potential. This study report for the first time about the anticancer and anti-inflammatory properties of G. xanthochymus whole fruit.
AB - Pro-oxidants play a crucial role in cancer by causing oxidative stress that leads to apoptosis. The present study demonstrates the prooxidant and anti-inflammatory potential of ethyl acetate and methanolic extracts of Garcinia xanthochymus fruit. Oxidation of Trolox and NADH activity indicated the pro-oxidant capacity of the extracts. Significant decrease in cell viability in B16F10 and MDA-MB-231 cancer cell lines and significant increase in caspase 3 activity after treatment with extracts indicated pro-oxidant induced apoptosis. Pre-treatment with the extracts significantly inhibited ROS, reduced NO production, inhibited LPS-induced COX-2 and suppressed IL-6 and TNF-α expression. HRMS analysis showed the presence of compounds like biflavonoids, xanthones, phloroglucinols, benzophenones, etc. The fruit is rich in total phenolic and flavonoid contents, and have DPPH radical scavenging, ferric reducing antioxidant and metal chelating potential. This study report for the first time about the anticancer and anti-inflammatory properties of G. xanthochymus whole fruit.
KW - antiproliferative
KW - biflavonoids
KW - cytokines
KW - Gamboge fruit
KW - reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=85207508901&partnerID=8YFLogxK
U2 - 10.1080/14786419.2024.2419492
DO - 10.1080/14786419.2024.2419492
M3 - Article
C2 - 39446993
AN - SCOPUS:85207508901
SN - 1478-6419
JO - Natural Product Research
JF - Natural Product Research
ER -
