TY - JOUR
T1 - Properties of Glycogen Synthetase in Erythrocytes
AU - Moses, Shimon W.
AU - Bashan, Nava
AU - Gutman, Alisa
PY - 1972/1/1
Y1 - 1972/1/1
N2 - Erythrocyte glycogen synthetase activity was found to be absolutely dependent on the presence of glucose‐6‐phosphate (Glc‐6‐P). The Km, for UDPG and the Ka for Glc‐6‐P were not altered by preincubation of the enzyme. Glucose‐6‐phosphate protected the enzyme against thermal in‐act:ivation. Enzyme activity was inhibited by P1, SO4=, ATP, theophylline and caffeine. Galactose‐6‐phosphate could replace Glc‐6‐P as activator. A study to the effect of various combinations of Glc‐6‐P, ATP and P1 at concentrations prevailing in the red blood cell suggested that Glc‐6‐P concentration, rather than the effect of inhibitors, was the main factor limiting the activity of erythrocyte glycogen synthetase. The low rate of glycogen synthesis in erythrocytes can be related to the lack of conversion of the enzyme to a glucose‐6‐phosphate independent form.
AB - Erythrocyte glycogen synthetase activity was found to be absolutely dependent on the presence of glucose‐6‐phosphate (Glc‐6‐P). The Km, for UDPG and the Ka for Glc‐6‐P were not altered by preincubation of the enzyme. Glucose‐6‐phosphate protected the enzyme against thermal in‐act:ivation. Enzyme activity was inhibited by P1, SO4=, ATP, theophylline and caffeine. Galactose‐6‐phosphate could replace Glc‐6‐P as activator. A study to the effect of various combinations of Glc‐6‐P, ATP and P1 at concentrations prevailing in the red blood cell suggested that Glc‐6‐P concentration, rather than the effect of inhibitors, was the main factor limiting the activity of erythrocyte glycogen synthetase. The low rate of glycogen synthesis in erythrocytes can be related to the lack of conversion of the enzyme to a glucose‐6‐phosphate independent form.
UR - http://www.scopus.com/inward/record.url?scp=0015406986&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1972.tb02088.x
DO - 10.1111/j.1432-1033.1972.tb02088.x
M3 - Article
C2 - 4197300
AN - SCOPUS:0015406986
SN - 0014-2956
VL - 30
SP - 205
EP - 210
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 2
ER -