Prostaglandin and prostamide concentrations in amniotic fluid of women with spontaneous labor at term with and without clinical chorioamnionitis

Hassendrini N. Peiris, Roberto Romero, Kanchan Vaswani, Nardhy Gomez-Lopez, Adi L. Tarca, Dereje W. Gudicha, Offer Erez, Eli Maymon, Sarah Reed, Murray D. Mitchell

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Objective: Prostaglandins (PGs) are considered universal mediators for the process of physiological parturition. This is based on observations that amniotic fluid concentrations of PGs are elevated prior to and during the onset of labor (mostly utilizing immunoassays). Distinguishing PGs from similarly structured molecules (i.e. prostamides; PG-EA) is difficult given the cross-reactivity of available antibodies and the chemical similarity between these compounds. Herein, this limitation was overcome by utilizing mass spectrometry to determine PG and PG-EA concentrations in amniotic fluid of women with spontaneous labor at term and in those with clinical chorioamnionitis (CHAM), the most common infection-related diagnosis made in labor and delivery units worldwide. Study Design: Liquid chromatography-tandem mass spectrometry (LC MS/MS) was used to determine the PG and PG-EA content in amniotic fluid samples of women with spontaneous labor at term with (n = 14) or without (n = 28) CHAM. Controls included women who delivered at term without labor (n = 10). Results: PGE2, PGF, and 13,14-dihydro-15-keto-PGF (PGFM) were higher in amniotic fluid of women with spontaneous labor at term than in those without labor. PGE2, PGF, and PGFM were also higher in amniotic fluid of women with CHAM than in those without labor. However, PGE2-EA and PGF-EA were lower in amniotic fluid of women with CHAM than in those without CHAM. The ratios of PGE2 to PGE2-EA and PGF to PGF-EA were higher in amniotic fluid of women with spontaneous labor at term with or without CHAM than in those without labor; yet, the ratio of PGF to PGF-EA was greater in women with CHAM than in those without this clinical condition. Conclusions: Spontaneous labor at term with or without CHAM is characterized by elevated amniotic fluid concentrations of prostaglandins (PGE2, PGF, and PGFM) but not prostamides. Quantification of these products by LC MS/MSlc==may potentially be of utility in identifying their physiological functions relevant to parturition. Prostaglandins (PGs) are critical for the onset and progression of labor. Structural similarities of PGs and prostamides (PG-EA) prevents their specific identification by immunoassay. We utilized LC MS/MS to determine PG and PG-EA content in amniotic fluid (AF) of women with spontaneous labor at term with or without CHAM and women who delivered at term without labor. Higher aamniotic ffluid PG levels were observed in women with spontaneous labor with and without CHAM compared to women delivering without labor. PG-EA levels in amniotic fluid of women with spontaneous labor and CHAM were lower than in women with spontaneous labor without CHAM but not those without labor. Ratios of PGs to PG-EAs were higher in AF of women with labor and CHAM compared to those without labor.

Original languageEnglish
Article number102195
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume163
DOIs
StatePublished - 1 Dec 2020
Externally publishedYes

Keywords

  • Eicosanoids
  • Intra-amniotic infection
  • Lipid mediators
  • Mass spectrometry
  • Parturition
  • Pregnancy
  • Prostaglandins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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