Prostaglandin E2 can bimodally inhibit and stimulate the epididymal adipocyte adenylyl cyclase activity

Rivka Cohen-Luria, Gilad Rimon

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Measurements of prostaglandin E2 (PGE2)-induced adenylyl cyclase acivity in membranes isolated from epididymal rat adipocytes revealed inhibition of cAMP production at low concentrations of PGE2 (<10 mM) and stimulation at higher concentrations. This biphasic effect of PGE2 was obtained when adenylyl cyclase was stimulated with GTP or NaF. In the presence of forskolin only the inhibitory phase by PGE2 was observed. Suplprostone, a PGE2 analogue, did not affect cAMP synthesis in the presence of either GTP or NaF; however, in the presence of forskolin, it inhibited cAMP production similarly to PGE2. Treatment of the membranes with cholera or pertussis toxin did not alter the biphasic effect of PGE2 on cAMP production. These findings raise the possibility that PGE2 acts through several receptor subtypes which are coupled to GTP binding proteins different from the classical Gi or Gs proteins.

Original languageEnglish
Pages (from-to)331-335
Number of pages5
JournalCellular Signalling
Volume4
Issue number3
DOIs
StatePublished - 1 Jan 1992

Keywords

  • Prostaglandin receptor
  • adenylyl cyclase
  • epididymal adipocyte membrane
  • forskolin
  • sulprostone

ASJC Scopus subject areas

  • Cell Biology

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