Abstract
Explants from rat antral mucosae in organ culture were exposed to prostaglandin E1 (2.8 × 10-5M) for 12 h. Prostaglandin E1 significantly stimulated the formation of cyclic AMP (pmol/mg tissue ± S.E.M.): 17.4 ± 2.5 in prostaglandin-treated samples compared to 5.5 ± 0.3 in control specimens. The increase in tissue cyclic AMP was not associated with enhanced release of immunoreactive gastrin into the culture media. Addition of isoproterenol (1 × 10-5M) to the bathing media resulted in a 4-fold increase in antral cyclic AMP production. In contrast to prostaglandin E1, isoproterenol exerted a marked stimulatory action on gastrin secretion during a 12 h culture period. Mucosal explants cultured for variable periods of time (6-12 h) in the presence of 8-Br-cyclic AMP (5mM) responded with a 2-fold rise in gastrin release. The results suggest that prostaglandin E1 enhances the level of cyclic AMP in sequestered cellular compartments not functionally linked to gastrin secretory mechanisms.
Original language | English |
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Pages (from-to) | 186-192 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 108 |
Issue number | 1 |
DOIs | |
State | Published - 16 Sep 1982 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology