Prostaglandin E1-stimulable cyclic AMP formation from rat gastric antral organ culture: Lack of effect on gastrin secretion

S. A. Lamprecht, J. Krawiec, B. Schwartz, P. Krugliak, J. Goldstein, H. S. Odes

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Abstract

Explants from rat antral mucosae in organ culture were exposed to prostaglandin E1 (2.8 × 10-5M) for 12 h. Prostaglandin E1 significantly stimulated the formation of cyclic AMP (pmol/mg tissue ± S.E.M.): 17.4 ± 2.5 in prostaglandin-treated samples compared to 5.5 ± 0.3 in control specimens. The increase in tissue cyclic AMP was not associated with enhanced release of immunoreactive gastrin into the culture media. Addition of isoproterenol (1 × 10-5M) to the bathing media resulted in a 4-fold increase in antral cyclic AMP production. In contrast to prostaglandin E1, isoproterenol exerted a marked stimulatory action on gastrin secretion during a 12 h culture period. Mucosal explants cultured for variable periods of time (6-12 h) in the presence of 8-Br-cyclic AMP (5mM) responded with a 2-fold rise in gastrin release. The results suggest that prostaglandin E1 enhances the level of cyclic AMP in sequestered cellular compartments not functionally linked to gastrin secretory mechanisms.

Original languageEnglish
Pages (from-to)186-192
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume108
Issue number1
DOIs
StatePublished - 16 Sep 1982
Externally publishedYes

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