Prostaglandin E2 in Previtellogeic Ovaries of the Prawn Macrobrachium rosenbergii: Synthesis and Effect on the Level of cAMP

Amir Sagi; Julia Silkovsy; Sigal Fleisher-Berkovich; Abraham Danon; Reuben Chayoth

doi:10.1006/gcen.1995.1161

Prostaglandin E₂ in Previtellogeic Ovaries of the Prawn Macrobrachium rosenbergii: Synthesis and Effect on the Level of cAMP

Amir Sagi, Julia Silkovsy, Sigal Fleisher-Berkovich, Abraham Danon, Reuben Chayoth

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Elcosanoids are thought to play a role in the regulation of invertebrate reproduction, as they do in vertebrate systems. This was investigated using the previtellogenic ovary of the freshwater prawn Macyobrachium rosenbergii as a biological model. Concentrations of prostaglandin E₂ (PGE₂), assessed by means of radioimmunoassay, in the previtellogenic ovary (oocyte diameter 20-40 μm) were 32.4 ± 14.1 pg/mg ovary. Preincubation of the ovary with indomethacin (10 μM) inhibited PGE₂ synthesis by 43%. In addition, if indomethacin was added to the culture medium. cAMP levels decreased by 48%. When previtellogenic ovaries were incubated in vitro with PGE₂ (0.05 μg/ml medium and up), cAMP levels in the tissue homogenate sharply increased. The levels of cAMP rose most significantly (up to 10-fold) when 1-10 μg PGE₂/ml medium was applied. These results suggest that PGE₂, and possibly other prostaglandins, may play a role in the endocrine regulation of crustacean reproduction.

Original language	English
Pages (from-to)	308-313
Number of pages	6
Journal	General and Comparative Endocrinology
Volume	100
Issue number	3
DOIs	https://doi.org/10.1006/gcen.1995.1161
State	Published - 1 Dec 1995

ASJC Scopus subject areas

Animal Science and Zoology
Endocrinology

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10.1006/gcen.1995.1161

Cite this

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title = "Prostaglandin E2 in Previtellogeic Ovaries of the Prawn Macrobrachium rosenbergii: Synthesis and Effect on the Level of cAMP",

abstract = "Elcosanoids are thought to play a role in the regulation of invertebrate reproduction, as they do in vertebrate systems. This was investigated using the previtellogenic ovary of the freshwater prawn Macyobrachium rosenbergii as a biological model. Concentrations of prostaglandin E2 (PGE2), assessed by means of radioimmunoassay, in the previtellogenic ovary (oocyte diameter 20-40 μm) were 32.4 ± 14.1 pg/mg ovary. Preincubation of the ovary with indomethacin (10 μM) inhibited PGE2 synthesis by 43%. In addition, if indomethacin was added to the culture medium. cAMP levels decreased by 48%. When previtellogenic ovaries were incubated in vitro with PGE2 (0.05 μg/ml medium and up), cAMP levels in the tissue homogenate sharply increased. The levels of cAMP rose most significantly (up to 10-fold) when 1-10 μg PGE2/ml medium was applied. These results suggest that PGE2, and possibly other prostaglandins, may play a role in the endocrine regulation of crustacean reproduction.",

author = "Amir Sagi and Julia Silkovsy and Sigal Fleisher-Berkovich and Abraham Danon and Reuben Chayoth",

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AU - Sagi, Amir

AU - Silkovsy, Julia

AU - Fleisher-Berkovich, Sigal

AU - Danon, Abraham

AU - Chayoth, Reuben

PY - 1995/12/1

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N2 - Elcosanoids are thought to play a role in the regulation of invertebrate reproduction, as they do in vertebrate systems. This was investigated using the previtellogenic ovary of the freshwater prawn Macyobrachium rosenbergii as a biological model. Concentrations of prostaglandin E2 (PGE2), assessed by means of radioimmunoassay, in the previtellogenic ovary (oocyte diameter 20-40 μm) were 32.4 ± 14.1 pg/mg ovary. Preincubation of the ovary with indomethacin (10 μM) inhibited PGE2 synthesis by 43%. In addition, if indomethacin was added to the culture medium. cAMP levels decreased by 48%. When previtellogenic ovaries were incubated in vitro with PGE2 (0.05 μg/ml medium and up), cAMP levels in the tissue homogenate sharply increased. The levels of cAMP rose most significantly (up to 10-fold) when 1-10 μg PGE2/ml medium was applied. These results suggest that PGE2, and possibly other prostaglandins, may play a role in the endocrine regulation of crustacean reproduction.

AB - Elcosanoids are thought to play a role in the regulation of invertebrate reproduction, as they do in vertebrate systems. This was investigated using the previtellogenic ovary of the freshwater prawn Macyobrachium rosenbergii as a biological model. Concentrations of prostaglandin E2 (PGE2), assessed by means of radioimmunoassay, in the previtellogenic ovary (oocyte diameter 20-40 μm) were 32.4 ± 14.1 pg/mg ovary. Preincubation of the ovary with indomethacin (10 μM) inhibited PGE2 synthesis by 43%. In addition, if indomethacin was added to the culture medium. cAMP levels decreased by 48%. When previtellogenic ovaries were incubated in vitro with PGE2 (0.05 μg/ml medium and up), cAMP levels in the tissue homogenate sharply increased. The levels of cAMP rose most significantly (up to 10-fold) when 1-10 μg PGE2/ml medium was applied. These results suggest that PGE2, and possibly other prostaglandins, may play a role in the endocrine regulation of crustacean reproduction.

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